摘要
①目的 研究美洛宁对大鼠脑缺血再灌注后海马区神经细胞凋亡及学习记忆功能的影响。②方法 选用成年Wistar大鼠制成大脑中动脉缺血模型,将大鼠随机分为脑缺血组、美洛宁治疗组、假手术组。每组又分别划分成缺血再灌注后1、3、6、12、24、48、72及168h等8个时相组,另取12只作为正常对照组。美洛宁治疗组经致伤后,给予腹腔注射美洛宁30mg/(kg·d),直至各时相点处死;脑缺血组、假手术组及正常对照组在相同时间给予等量的生理盐水腹腔注射作为对照。各组均在相应的时间点处死取材进行原位细胞凋亡检测;另取48只大鼠随机分为脑缺血组、美洛宁治疗组、假手术组及正常对照组,进行水迷宫测试。③结果 美洛宁能够使海马区神经细胞凋亡的高峰明显下调,使水迷宫测试的潜伏期明显缩短。④结论 美洛宁能够有效抑制大鼠脑缺血再灌注后海马区神经细胞凋亡,明显改善学习记忆功能障碍。
Objective To investigate the effect of CTP on neural apoptosis and cognitive function after transient focal cerebral ischemia- reperfution (IR) in rats. Methods The model of transient focal cerebral ischemia- reperfution (IR) was established. 300 Wistar rats were divided randomly into IR group,CTP treating group and fake operation group and each of these three groups was divided into 8 subgroups,namely, K 3> 6,12, 24,48, 72,168 hours. At the same time,the rest 12 rats were taked as the normal group for comparison. The CTP treating group after injury were treated with CTP 30mg/(kg ?d) in peritoneum;IR group,the fake operation group and the normal group were treated with saline for comparison at the same time and all were killed at each time point,then apoptosis in situ was detected. Another 48 rats were divided randomly into 4 groups,namely .IRgroup,CTP treating group, fake operation group and normal group,and their cognitive dysfuction was evaluated using the morris water maze (MWM). Results CTP can decrease obviously the apex of apoptosis-positive cells in hippocampus and shorten the delitescence of morris water maze tests after IR. Conclusions CTP may suppress effectively apoptosis of nerve cells after IR and improve cognitive function.
出处
《华北煤炭医学院学报》
2002年第5期559-560,共2页
Journal of North China Coal Medical College
