摘要
目的探讨丙泊酚对大鼠肝脏缺血再灌注损伤时Bcl-2和P53表达的影响及其保护作用。方法健康成年雄性SD大鼠54只,随机分为3组:假手术组(Ⅰ组)、缺血再灌注组(Ⅱ组)、丙泊酚组(Ⅲ组)。每组根据再灌注时间不同又分为3个时间段(1、2、3h)。制作70%肝脏缺血再灌注模型,实验结束后即刻取肝左叶做标本。用免疫组织化学法检测Bcl-2与P53蛋白表达量,原位细胞凋亡检测(TUNEL)法检测肝细胞凋亡指数(AI),光镜下观察肝组织的病理学变化。结果与Ⅰ组比较,Ⅱ、Ⅲ组肝组织Bcl-2、P53蛋白含量、凋亡指数差异均有统计学意义(P<0.05);与Ⅱ组比较,Ⅲ组Bcl-2蛋白表达增加、P53蛋白表达和AI减少(P<0.05);光镜下Ⅲ组的肝细胞病理学变化轻于Ⅱ组。结论丙泊酚可以通过调节Bcl-2、P53蛋白表达,使肝细胞凋亡减轻,从而对大鼠缺血再灌注肝脏有一定的保护作用。
Objective To investigate the effect of propofol on cell apoptosis induced by hepatic ischemia-reperfusion in rats. Methods Fifty-four SD rats were randomly divided into 3 groups （n = 18） ：sham-operated control group （group 1 ）, isehemia-reperfusion group （group Ⅱ ）and propofol group （group Ⅲ ）. Each group was divided into 3 time phases at 1 h, 2 h, 3 h after reperfusion to establish a rat model of hepatic ischemia-reperfusion. The left hepatic lobes were sampled at the end of reperfusion. Bcl-2 and P53 protein expression in liver tissue was assessed by immunohistochemical method. The apoptosis index （AI） of hepatic cell were measured by TUNEL method. The histological change of liver were observed using light microscopy. Results Bcl-2, P53 protein expression and apoptosis index of hepatic cell were statistically different between group Ⅰ, group Ⅱ and Ⅲ （ P 〈 0.05）. Bcl-2 protein expression was significantly higher while P53 protein expression and AI significantly lower in group Ⅲ than those in group Ⅱ （P 〈 0.05）. The pathological lesions were lighter in group Ⅲ than those in group Ⅲ under light microscope. Conclusion Propofol can relieve the liver cellular apoptc6is by regulating Bcl-2 and P53 protein expression. It may protect rats from hepatic isehemia-reperfusion injury.
关键词
丙泊酚
肝
再灌注损伤
凋亡
Propofol
Liver
Reperfusion injury
Apoptosis
