摘要
目的 探讨牙骨质基质提取物同 型胶原蛋白及纤维粘连蛋白 (FN )的关系 .方法 制取牙骨质基质胍提取物 ,分别以含 型胶原蛋白酶及 FN多克隆抗体预处理后的牙骨质基质提取物浓度为 10 mg· L- 1的 DMEM培养液为实验组 ,以同浓度牙骨质基质提取物的 DMEM培养液为阳性对照组 ,以不含牙骨质基质提取物 DMEM培养液为阴性对照组 ,测牙龈成纤维细胞和成骨细胞在上述 4组培养液中培养作用 30 ,6 0 ,90和 12 0 min后的细胞贴壁率 .结果 型胶原蛋白酶组在作用 90 min后 ,同阳性对照组相比 ,牙龈成纤维细胞的贴壁率下降了 15 % (P<0 .0 5 ) ,成骨细胞的贴壁率下降了 11% (P<0 .0 5 ) .FN-多克隆抗体组同阳性对照组相比 ,在 30 ,6 0 ,90和 12 0 min各时间点两组牙龈成纤维细胞和成骨细胞贴壁率的变化无显著差异 (P>0 .0 5 ) .结论 胶原蛋白存在于牙骨质基质提取物中 ,但不是其主要活性成分 ;牙骨质基质提取物内的活性蛋白是不同于成纤维 FN的其他粘附蛋白 .
AIM To study the effects of Ⅰ collagenase and polyclonal antibody to fibronectin on bioactivity of cementum extracts from attachment of gingival fibroblasts and osteoblasts. METHODS Cementum extract (CA) by guani dine was obtained. Human gingival fibroblasts and MC3T3 E1 osteoblasts were incubated in the following four solutions: 1. DMEM; 2. DMEM+10 mg·L -1 CA; 3. DMEM+10 mg·L -1 CA+ 5 mg·L -1 Ⅰ collagenase; 4. DMEM+10 mg·L -1 CA+polyclonal antibody to fibronectin. The attachment rate of the cells at different incubation time points (30, 60, 90 and 120 min) was measured. RESULTS Compared with that of Group 2, the cell attachment rate of fibroblasts and osteoblasts in Group 3 declined by 15% ( P < 0.05 ) and 11% ( P <0.05) respectively when the cells had been incubated for 90 min. But the difference in cell attachment rate between Group 2 and Group 4 was not significant. CONCLUSION Ⅰ collagen is one of the active components of the human cementum extract but not the major one. The major active component is different from fibronectin.
出处
《第四军医大学学报》
北大核心
2002年第17期1624-1626,共3页
Journal of the Fourth Military Medical University
