摘要
目的 :探讨制取牙骨质基质提取物的可行性并分析提取物的蛋白组分。方法 :收集因正畸而拔除的牙周未感染的恒牙 ,用钝性刮匙去除牙根表面的结缔组织 ,在解剖显微镜下从牙本质表面刮下牙骨质 ,分别制取牙骨质基质醋酸和胍 2种提取物。采用SDS 聚苯烯酰胺凝胶电泳法分析 2种提取物的蛋白组分。结果 :牙骨质基质醋酸提取物主要含有Mr 5 5 0 0 0和Mr 680 0 0 2种蛋白 ,而牙骨质基质胍提取物则含Mr 62 0 0 0、Mr60 0 0 0、Mr 5 5 0 0 0及Mr 410 0 0 4种蛋白。结论
Objective: To prepare human cementum extract and to assess its protein content. Methods: Human permanent teeth were collected from the oral surgery clinic. Cementum was obtained from freshly extracted healthy teeth without inflammation.Connective tissue adhereing to the root surfaces was removed, and cementum was harvested with a curette. Then the cementum was treated by CH 3COOH and guanidine respectively to obtain two extracts. Protein content was assessed by sodium dodecyl sulphate ( SDS) polyacrylamide electrophoresis. Results: Extract obtained from CH 3COOH treatment consisted of two kind of proteins ( M r 55 000 and M r 68 000); that of guanidine consisted of four kind of proteins ( M r 62 000, M r 60 000 , M r 5 5000 and M r 41 000).Conclusion: The methods used is an applicable way to prepare cememtum extracts.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2002年第3期195-197,共3页
Journal of Practical Stomatology
