摘要
目的 研制一种新型神经组织工程修复材料基体具有轴向孔隙胶原 -氨基聚糖基质的制备方法 ,用于节段性脊髓及周围神经损伤的修复 .方法 将注有胶原 -硫酸软骨素 - 6悬浊液的硅胶管逐步浸入 - 180℃低温液氮中、浸入速度控制在 5× 10 - 5m· s- 1～ 10× 10 - 7m· s- 1之间 ,冷凝成冰晶圆柱 ,冻干 ,通过显微物镜、电镜扫描观察不同浸入速度所制胶原 -氨基聚糖基质内部孔排列规律及走行方向 ,测量孔的大小 .结果 在 <5× 10 - 5m· s- 1 速度时 ,胶原 -氨基聚糖基质微孔走向多呈与圆柱轴的方向相一致的规律排列 ,孔径随速度减慢而逐步减小 .结论 所研制的胶原 -氨基聚糖基质具有不同直径轴向微管结构特性 ,可作为神经组织工程材料基体用于节段性脊髓及周围神经损伤的修复 .
AIM To develop a new type nerve repair material with collagen glycosaminoglycan matrix by tissue engineering to treat the segmental defect of spinal cord and peripheral nerve. METHODS The silicon pipe filled with collagen chondroitin sulfate 6 suspension was dipped gradually into the liquid nitrogen (-180℃). The dipping speed was controlled exactly between 5×10 -5 m·s -1 to 10×10 -7 m·s -1 . The suspension was frozen and lyophilized. The micro structure of the material was observed by optical microscope and scanning electron microscope and the size of the micropores was measured. RESULTS A regular channel was found as one direction in the material when the speed was below 5×10 -5 m·s -1 and the size of the channel was closely related with the dipping speed, the slower dipping and the smaller channel. CONCLUSION The nerve tissue engineering material with channels of different size has a reasonable micro structure of one way direction, which can be used in the treatment of segmental spinal cord and peripheral nerve defect in the future.
出处
《第四军医大学学报》
北大核心
2002年第17期1574-1576,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3 0 0 70 761)
国家高等院校骨干教师资助项目
关键词
神经组织工程
生物材料
脊髓
周围神经损伤
nerve tissue engineering
biomaterial
spinal cord
peripheral nerve injuries
