摘要
本实验抽取小鼠腹腔渗出细胞,体外培养,用荧光测定法检测腹腔巨噬细胞释放的 H_2O_2量。结果表明 CCl_4所致肝损伤小鼠腹腔单核巨噬细胞数和释放 H_2O_2能力明显下降,猪苓多糖能增加和回升正常及肝损伤小鼠的腹腔巨噬细胞数量和释放 H_2O_2能力。该药能提高机体的细胞免疫功能,是治疗慢性肝炎的重要机理之一。
The cells in peritoneal cavities of mice were taken out and cultured in vitro.The amount to release H_2O_2,of the macrophages was assayed by fluorimetry.Polyporus umbellatus polysaccharide (PUP)could not only increase the number of macrophages and the amount of H_2O_2 release in the peritoneal cavities of normal mice.but also raise the lowered munber of macrophages and the ability to release H_2O_2 in the peritoneal cavities of the mice with liver lesions caused by CCl_4significantly. So.PUP could improve the cellular immunity of normal mice and the mice with liver lesions.
关键词
猪苓多糖
肝损伤
单核巨shi细胞
Polyporus umbellatus polysaccharide
liver lesions
macrophages of peritoneal cavities
