摘要
线粒体结合ATPase及质膜和液泡膜结合ATPase活性随花生种子吸胀的起始逐渐增加,酶活性与种子活力有正相关性。以萌发一周的花生下胚轴为实验材料,线粒体ATPase,质膜ATPase和液泡膜ATPase的反应最适pH均为9.在pH 6～10范围内随pH的升高H^+分泌增多。膜ATP酶为二环已烷亚胺抑制,而为KCl激活;NaN_3特异地抑制线粒体ATP酶,而NaVO_3专一地抑制质膜ATP酶,但两者对液泡膜ATP酶均无效。Mg^(++)、K^+激活ATPase,也刺激H^+分泌。质膜ATPase抑制剂NaVO_3对H^+9分泌作用不大,而线粒体ATPase抑制剂NaN_3及解偶联剂DNP均显著抑制H^+分泌。CoⅠ刺激H^+分泌而C_oⅡ抑制H^+分泌。似乎线粒体ATP产量对H^+分泌更重要。
Accompanying the imbibition,the activity of mitochondrial ATPaseand plasma,-tonoplast membrane-ATPase increased.Seed vigour was positively correlated to the activity of membrane bound ATPase.The results we gained indicated that the optimum pH of mitochondrial ATPase,plasma membrane ATPase and tono-plast membrane ATPase from embryonic axes were all pH9.Activities of mitochon-drial,plasma-and tonoplast membrane ATPase were inhibited conspicuously by dicyclohexylcarbodiimide(DCCI),while they were accelerated by KCI.NaN_3 is a
special inhibitor of mitochondrial ATPase activity and NaVO_3 is also a particμlar inhibitor of plasmamembrane ATPase activity,but both of them have almost no inhibitory effect on tonoplast-membrane ATPase activity.H^+ extrusion increased with the rise of pH in pH6-9;Mg^(2+) and K^+ stimulated the H^+ net efflux;it was hardly to see the effect of the special inhibitor of plasma membrane ATPase--NaVO_3 on H^+ efflux,while the mitochondrial ATPase inhibitor--NaN_3 and the
uncoupling factor DNP inhibited the H^+ extrusion strongly;CoI activated the H^+extrusion and CoII had repressed effect on the H^+ efflux.These results seemed to suggest that the ATP production of mitochondria may play an important role in the H^+ extrusion.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
1991年第4期100-106,共7页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金
