摘要
采用十二烷基硫酸钠 聚丙烯酰胺凝胶不连续垂直板电泳 (SDS PAGE)对 16 2头二花脸母猪乳中一组高分子量蛋白质 (HMWP)进行了检测 ,计算了该位点的基因型频率、等位基因频率和遗传多样性指数等 ,并运用线性模型统计方法分析了该位点的不同基因型与母猪繁殖性能的关系。结果表明 ,在二花脸猪种群 ,乳中HMWP存在B(M :11480 0± 40 0 )和D(M :10 140 0± 6 0 0 )两种带 ,分别受二个等位基因HB 和HD 控制。BB、BD和DD三种基因型频率分别为 0 6 6 6 7、0 2 716和 0 0 6 17,两个等位基因HB 和HD 频率分别为 0 80 2 5和 0 1975 ,遗传多样性指数为0 3170。在三种HMWP基因型中 ,不同基因型母猪的繁殖性能在活仔数和窝重上存在着显著差异 (P <0 0 5 ) ,但在初生仔猪个体重和 2 0日龄窝重上未达到显著水平 (P >0 0 5 )。
High molecular protein (HMWP) polymorphism of milk was investigated in 162 Erhualian sows with SDS-PAGE.The genotypic frequencies,allele frequencies and heterozygosity(h)in the locus of HMWP were calculated.The relationship between HMWP polymorphism and reproductive performance was analyzed with linear model.The results showed that there were B(M:114800±400) and D (M:101400±600) protein types in Erhualian sow milk,the HMWP locus was controlled by two alleles,H B and H D.The frequencies of genotype BB,BD and DD were 0.6667,0.2716 and 0.0617 respectively,and the frequencies of allele B and D were 0.8025 and 0.1975 respectively.The heterozygosity(h)of this locus was 0.3170.The effects of HMWP genotypes as a discrete variable were significant for litter weight and number of piglets born alive (P<0.05),but not significant for weight of newborn piglet and litter weight at 20 day(P>0.05).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第5期429-432,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目 ( 39770 5 5 4)
