摘要
目的 探讨 5 -脂氧合酶抑制剂菲尼酮对大鼠脑损伤后脑循环的影响。 方法 实验分为对照组和给药组。制备流体冲击致大鼠脑损伤模型 ,用氢清除法和二胺基联苯胺染色及图像观察分析大鼠海马血流量 (hippocampalbloodflow ,HBF)和血管密度变化以及菲尼酮对其的影响。结果 大鼠脑损伤后 1hHBF开始下降 ,4h达最低 ,8h以后逐渐恢复 ;与此同时海马血管密度在伤后 4h升高达峰值 ,8h后逐渐下降至伤前水平。给药组在伤后 8h海马血流量已恢复到伤前水平 ,海马血管密度在伤后 4h有增加的趋势 ,但与伤前比较 ,差异无显著性意义。 结论 流体冲击致大鼠脑损伤后 ,菲尼酮可不同程度地逆转HBF降低与血管密度破坏性的增加 ,这可能与其抑制了炎性介质如白三烯的产生等有关 ,从而有利于创伤后脑循环功能的恢复。
Objective To explore the effects of phenidone on cerebral circulation following experimental brain injury in rats. Methods The animals were divided into control group and treatment group. The fluid percussion brain injury model in the rat was made. Changes of regional blood flow and angioarchitecture area density (AAD) in hippocampus were determined by hydrogen clear method and diaminobenzidine cytochemical assay respectively. The effect of phenidone on hippocampus circulation was synchronously investigated. Results The hippocampus blood flow began to decrease 1 hour after injury, reached the minimum at the 4th hour. And 8 hours after injury, it returned gradually, while hippocampus AAD peaked at the 4th hour after injury and declined to pre-injury level 8 hours after injury. In the treatment group, at the 8th post-injury hour, AAD returned to the pre-injury level, at the 4th hour, the hippocampus vascular density proved to increase. However, it was not remarkably different from the control group. Conclusions Phenidone reverses the declined hippocampus blood flow and the destructively elevated AAD to a degree after fluid percussion rat brain injury, which may be associated with inhibition of inflammatory mediators and therefore contribute to the recovery of brain circulatory function.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2002年第9期544-546,共3页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目 (3 93 70 689)
关键词
菲尼酮
大鼠
脑损伤
脑微循环
脂氧合酶抑制剂
脑血管密度
Brain injuries
Lipoxygenase inhibitors
Regional blood flow, hippocampus
Cerebrovascular density
