摘要
应用血细胞中内源性过氧化物酶将底物过氧化氢分解,产生原子氧,使无色联苯胺及其衍生物呈色,显示中枢神经系统微血管的原理,在辣根过氧化物酶(HRP)呈色技术启示下,确立了4种中枢神经系统微血管显示法,即二氨基联苯胺法(DAB)、二盐酸联苯胺法(BDHC)、邻-联茴香胺法(O-D)以及四甲基联苯胺法(TMB)。实验结果表明,这4种方法均能清晰地呈现中枢神经系统内的徽血管,4种方法的显示结果并无显著性差异(P>0.05)。这4种方法的建立将为中枢神经系统微血管解剖学研究,病理组织血管构筑研究以及中枢神经系统内移植物血管重建研究提供了重要的技术手段。
Angioarchiteture of the CNS were visualized with peroxidase cytochemistry in rat. This technique can successfully demonstrat that the endogenous peroxidase activity of red cells and granular leukocytes(especilly polymorphonuclear and eosinophilic)were excellent mar- kers for revealing CNS blood vessels at the light microscopic level when 3,3′-diaminobenzi- dine (DAB), benzidine dihydrochloride (BDHC), O-dianisidine (O-D) and tetramethyl- benidine(TMB) were used as chromogen. The four peroxidase cytochemical approches are useful for visualizing CNS microvasculature in nomal laboratory animals; the four approches have potential application to cerebrovascular studies of stroke, trauma, neoplasia and intra- cerebral grafts.
关键词
过氧化物酶
联苯胺
中枢神经系统
peroxidase
benzidine
central nervous system
angioarchtecture
