摘要
目的 :建立反相高效液相色谱法测定关木通水煎剂中马兜铃酸A的含量。方法 :色谱柱 :SpherisorbODS2(4 6mm×250mm ,5μm ) ;流动相 :甲醇 -水 -冰醋酸 (72∶27∶1) ;流速 :1 0ml/min ;检测波长 :250nm。结果 :马兜铃酸A在0 0128μg～0 4096μg范围内线性关系良好 ,回归方程 :Y=4553 7 +5388319 3X ,r=0 9999 ;平均回收率为97 33 % ,RSD为2 34 %。结论 :本方法简便、灵敏、准确。
OBJECTIVE:To establish a RP-HPLC method for the determination of aristolochic acid A in decoction of Caulis Aristolochiae Manshuriensis METHODS:The analytical column was Spherisorb ODS2 column(4 6mm×250mm,5μm) The mobile phase consisted of a mixture,methanol-water-acetic acid(70∶27∶1) The flow rate was 1 0ml/min The UV detection wavelength was 250nm RESULTS:The linear range was 0 0 128μg～0 4 096μg(r=0 9 999) The regression equation was Y=4 553 7+5 388 319 3X The average recovery of aristolochic acid A was 97 33%(RSD=2 34%) CONCLUSION:This method is simple,sensitive and accurate
出处
《中国药房》
CAS
CSCD
2002年第9期551-552,共2页
China Pharmacy
基金
广东省中医药管理局科研基金资助课题(101035)
