摘要
目的 探讨兔骨膜成骨细胞 (RPOB)和肾血管内皮细胞 (RRVEC)不同比例下间接共培养对成骨细胞增殖及功能的影响 ,优选细胞间接共培养的适宜比例。方法 取 RPOB和 RRVEC,采用细胞嵌盒培养系统 ,对照组、试验 1、2、3组分别以 RPOB和 RRVEC按 1∶ 0、2∶ 1、1∶ 1和 1∶ 2间接共培养 4天。通过细胞计数、碱性磷酸酶 (AL P)活性测定及 3H-脯氨酸掺入试验观察 RPOB和 RRVCE增殖分化能力及功能状态。结果 试验 1组 RPOB较对照组、试验2、3组增殖活跃 (P<0 .0 5 ) ,且 3H-脯氨酸掺入较高 (P<0 .0 5 ) ;试验 1组 AL P活性较对照组和试验 3组高 (P<0 .0 5 ) ,但与试验 2组无显著差异 (P>0 .0 5 )。结论 RPOB和 RRVEC以 2∶ 1进行间接共培养时 RPOB增殖能力强、功能活跃 。
Objective To determine an optimal co culture ratio of the rabbit periosteal osteoblasts (RPOB) and rabbit renal vascular endothelial cells(RRVEC) without direct contact for future study of bone tissue engineering. Methods RPOB and RRVEC in the ratios of 1∶0(control group), 2∶1(group 1), 1∶1(group 2) and 1∶2(group 3) were co cultured by six well plates and cell inserts. Four days later, the proliferation of RPOB and RRVEC were examined through cell count. Differentiated cell function was assessed by alkaline phosphatase (ALP) activity assay and 3H proline incorporation assay. Results When RPOB and RRVEC were indirectly co cultured , the proliferation of RPOB and 3H proline incorporation was higher in group 1 than in the other experimental groups and control group ( P< 0 05). ALP activity of RPOB was higher in group 1 than in control group and group 3 ( P< 0 05), but there was no significant difference between group 1 and group 2 ( P> 0 05). Conclusion These results suggest that RPOB and RRVEC co cultured in a ratio of 2∶1 is optimal for future study of bone tissue engineering.
出处
《中国修复重建外科杂志》
CAS
CSCD
2002年第5期307-310,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展规划 (863) (2 0 0 1 AA2 1 60 1 1 )
国家自然科学基金重点资助项目 (39830 1 0 0 )~~
