摘要
通过对红鲤、红鲫、镜鲤、鲤鲫杂种二倍体一代 ,二代 ,鲤鲫杂种三倍体 ,鲤鲫复合三倍体 ,鲤鲫杂种四倍体一代 ,二代的同工酶及蛋白电泳谱型和扫描数据分析表明 :在鲤鲫人工多倍体谱系中 ,亲代的等位基因在杂交子代中共有四种表达模式 :( 1 )两亲本基因在子代中共同表达 ,即共显表达 ;( 2 )父本的基因表达受到部分或完全的抑制 ,即母本的基因优先得到表达 ;( 3)母本的基因表达受到抑制 ,父本的基因得到表达 ;( 4 )双亲本的基因表达均受到一定程度的抑制或都不表达。其中第一种表达模式是主要的模式。根据以上基因在杂交子代中的表达特点 ,可用同工酶和蛋白电泳图谱将鲤鲫人工多倍体谱系的各种生物型逐一加以区分。
Analysis and comparisons of expression patterns of isozymes and proteins in artificial polyploid lineage among hybrids between common carp and crucian carp, including red carp, red crucian carp, mirror carp, diploid hybrid between common carp and crucian carp, gynogenetic diploid hybrid, triploid hybrid, multiple triploid hybrid, allotetraploid and its second generation, have been carried. Results revealed that there were 4 expression modes of parental alleles in their offspring: (1) simultaneous expression of both parental alleles, (2) expression of paternal alleles was inhibited, but maternal alleles were preferentially expressed, (3) expression of maternal alleles was inhibited, but paternal alleles were preferentially expressed, (4) alleles of two parental were inhibited to a certain extent or were not expressed completely. Among these 4 expression models the mode (1) was the principal model. According to these gene expression modes, different biotypes of this lineage including diploid, triploid and tetraploid hybrids could be distinguished from each other and from their original parents easily.
出处
《水生生物学报》
CAS
CSCD
北大核心
2002年第5期425-432,共8页
Acta Hydrobiologica Sinica
基金
国家自然科学基金重点项目 (3 982 3 0 0 3 )
国家自然科学基金项目 (3 0 1 70 73 8)资助
