摘要
用免疫组织化学和聚丙烯酰胺凝胶同工酶电泳方法研究了小鼠肾在热休克(46℃,30分钟)恢复期(4h和12h)HSP70的表达和乳酸脱氢酶(LDH)同工酶的变化。结果表明:(1)HSP70主要定位于肾小管上皮细胞胞质中,细胞核内未见表达;(2)HSP70免疫阳性反应在肾髓质较肾皮质强,肾小管较肾小球强;(3)热休克诱导小鼠肾LDH同工酶活性增强。提示:LDH同工酶可能对细胞热耐受性的建立有重要作用。
The immunochemical method and PAGE were used to analyze the changes of the expression of HSP70 and lactate dehydrogenase (LDH)isozymes of mouse kidney during the recovery period of heat shock(46t ,30min).The results showed that:(1)the HSP70 positive immunoreactivity was localized in the renal tubule epithelial cytoplasm, while the nucleus was negative; (2)The HSP70 positive im-munoreactivity of the renal medulla was stronger than that of the renal cortex, and the HSP70 positive immunoreactivity of the renal tubule was stronger than that of the renal corpuscle; (3)Heat shock induced increase in the activities of LDH isozymes in kidney. The results suggested that LDH isozymes may be play an important role in the building of heat tolerance of cells.
出处
《细胞生物学杂志》
CSCD
北大核心
2002年第4期242-245,共4页
Chinese Journal of Cell Biology
基金
中法先进技术合作项目(B96-11)
河南省动物学重点学科资助
