摘要
以之江菌素产生菌玫瑰黄链霉菌杭州亚种 93 -1 5-3 2菌株为出发菌 ,研究离子注入微生物的诱变效应。结果表明 ,各剂量N+离子注入之江菌素产生菌的总变异率达 42 4%～ 73 0 % ,其中正变异率为 5 8%～ 3 8 2 %。通过 9批诱变实验 ,筛选到 1株高产菌株 97 49,效价比出发菌株提高 4倍多。通过 5代传代 ,产素性能能稳定遗传 ,并通过了 40 0L发酵罐中试 ,表明离子注入处理是一种有潜力的微生物诱变育种新方法。
The biological effects of Streptomyces roseoflavus subsp.hangzhouensisn.Subsp.with N + implantation are reported.The total and postitive mutation rates with various doses(3×10 15 ～15×10 15 N +/cm 2)were 42 4%～73 0% and 5 8%～38 2%,respectively.Mutant 97\|49 with potency over four times more than that of the parental strain was obtained through N + implantation and showed good genetic stability.These results showed that ion implantation was an effective method for microbe mutagenesis.
出处
《核农学报》
CAS
CSCD
北大核心
2002年第4期208-211,共4页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金及国家九五攻关资助项目
