摘要
用台中 6 5 (粳稻 ) / ARC10 313(籼稻 )的重组近交系 (F1 0 )构建了 RFL P连锁图谱 ,含 113个分布均匀的标记。作成的图谱覆盖全基因组 ,全图总长 14 6 2 .4 c M,图中标记位置与所使用的参照图谱基本符合。利用该重组自交家系材料与亲本台中 6 5回交得到 BF1 家系 ,用于对小穗不育和花粉不育的 QTL 分析 ,检测出 3个小穗不育和 1个花粉不育QTL,且有一个小穗不育位点和花粉不育位点重叠于第 7条染色体的标记 R1789处。已知的恢复基因 Rf4与该座位对应。
Quantitative trait locus (QTL) analysis has been carried out to identify genes conferring spikelet sterility and pollen sterility in rice. As a first step, an RFLP linkage map based on the recombinant inbred lines (RILs) was constructed. 125 RILs (F 10 ) derived from a cross between a Japonica cultivar Taichung 65 and an Indica cultivar ARC10313 were developed. The RFLP map contained 113 well dispersed RFLP markers. Total map length was 1462.4 cM Linkage arrangement of the RFLP markers was in good agreement with that of the previously constructed maps. 63 backcross F 1 lines (BF 1) derived from RILs were used as a segregating population for QTL analysis. Three QTLs for F 1 spikelet sterility were detected on Chromosome 1, 7 and 11. Among them, a QTL near the RFLP marker R1789 (chromosome 7) was detected at significant level P= 0.0001, and one QTL for F 1 pollen sterility was also detected near R1789 marker. Rf4 on chromosome 7 is known as fertility restorer gene. Above QTLs detected on chromosome 7 in this study appeared to correspond to Rf4 locus.
出处
《作物学报》
CAS
CSCD
北大核心
2002年第5期650-653,共4页
Acta Agronomica Sinica
基金
"教育部优秀骨干教师基金"
国家留学基金委员会资助~~
