摘要
应用套式PCR检测了88例丙型肝炎患者血清和外周血单个核细胞(PBMC)中HCV RNA;结果发现:慢性丙肝患者PBMC中HCV RNA检出率显著高于急性丙肝患者(P<0.001);急、慢性丙肝患者血清和慢性丙肝患者PBMC中HCV RNA检出率显著高于ALT正常的抗-HCV阳性者(P<0.001);少数患者血清中HCV RNA阴性,PBMC中可测及,部分患者血清抗一HCV阴性,而血清HCV RNA阳性.提示:血清HCV RNA检测有助于抗-HCV阴性丙肝的诊断和早期诊断;丙肝的肝损害可能与HCV RNA血症有关;PBMC中HCV感染在丙肝的慢性化和慢性肝损害中可能起一定作用;PBMC中可贮存HCV RNA。
Hepatitis Cvirus(HCV)RNA was detected by nested-polymerase chain reaction(Nested-PCR)in serum and in peripheral blood mononuclear cells(PBMC)of 88 patients with hepatitis C(HC).It was found that the positive rate of HCV RNA in PBMC of patients with chronicHC was markedly higher than that of patients with acute HC(P<0.001);The pos-itive rates of HCV RNA in serum of patients with acute and chronicHCand in PBMC of patients with chronic HC was significantly higher than those of anti-HCV positive patients with normal ALT level(P<0.001):HCV RNA was negative in serum of 2 patients.but it could be detected in PBMC;In 12 patients anti-HC'V was negative while HCV RNA was positive in serum.These results suggest that:(1)detection of serum HCV RNA by nested-PCR might be helpful to the diagnoxis or early diagnosis of anti-HCV negative HC;(2)the liver damage in patients with HC might be correlated with HCV-viremia;(3)infection of HCV in PBMC might play an important role in chronic liver damage in patients with HCand chronicity of the clinical course;(4)PBMC might be considered as a"reservoir"of HCV.
出处
《空军总医院学报》
1996年第1期4-7,共4页
Journal of General Hospital of Air Force,PLA
