摘要
将一种新型硅烷偶联剂 β-(3 ,4-环氧环己基 )乙基三甲氧基硅烷 (β-ECTS)与辛酸反应 ,然后再键合到硅胶上 ,得到了酯型键合固定相 .用元素分析、 1 3C固体核磁共振谱、红外光谱进行了表征 .以甲醇和水为二元流动相 ,用包括碱性、酸性和中性有机化合物在内的混合物评价了该固定相的疏水性、选择性和亲硅醇基效应 ,并考察了该填料适用的 p H范围及水解稳定性 .结果表明 ,该固定相具有较好的色谱性能 ,且在 p H=2 .5～ 7.5之间稳定性良好 。
A novel ester bonded packing for reversed phase HPLC(RP HPLC) was synthesized by reacting octanoic acid with β (3,4 epoxycyclohexyl) ethyltrimethoxy silane and then the intermediate product was coupled onto porous silica. Characterization of the prepared packing was carried out with elemental analysis, solid state 13 C NMR, and Fourier transform infrared spectrometry. Hydrophobicity, selectivity and silanophilic activity of the packing were evaluated by using organic components including acidic, basic and neutral analytes as probe molecules and methanol water as binary mobile phase. The usage ranges of pH and stability of the packing were also evaluated. The results show that the novel stationary phase has excellent chromatographic properties and resist to hydrolysis between pH=2 5-7 5, it can be used for the separation of basic solutes efficiently.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第9期1684-1687,共4页
Chemical Journal of Chinese Universities
基金
中国分析测试协会科学仪器发展基金 (1999年 )资助
