摘要
目的：通过评价体外抑制人肝癌细胞（BEL-7402）活性,优化黄腐酸（FA）氧解制备工艺。方法：采用BOX-BehnKen Design法,以FA提取率及其体外抑制肿瘤细胞活性的IC50为指标,选取最优工艺。结果：应用BOX-BehnKen Design法制备FA的最佳条件为氧化剂浓度15%,氧解时间90 min,液固比3∶1。结论：BOX-BehnKen Design法稳定可靠,可用于氧解制备FA。
Objective：To optimize the preparation process of fulvic acid（FA） oxidation by evaluate the inhibition of human cell line BEL-7402 activity. Methods：Box-BehnKen design was used to optimize the technology with FA extraction rate with IC50 value（inhibiting cell line activity in vitro） as the indexes. Results： The optimal conditions for Box-Behnken design were as follow：the oxidant concentration was 15%,the extraction time was 90 minutes,and the liquid-solid ratio was 3：1. Conclusion：Box-Behnken design is stable and reliable,and can be used in the preparation of FA by oxidative degradation.
作者
刘金安
崔清华
张洪
李思齐
侯雪雯
王刚
刘帆
田景振
LIU Jin＇an;CUI Qinghua;ZHANG Hong;LI Siqi;HOU Xuewen;WANG Gang;LIU Fan;TIAN Jingzhen(Shandon~ University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《山东中医杂志》
2018年第11期943-948,共6页
Shandong Journal of Traditional Chinese Medicine
基金
山东省重点研发设计(重大关键技术及重点产业关键技术)(编号:2016CYJS08A01-8)
国家科技重大专项(编号:2017Z X09301030)
