摘要
目的探讨hsa_eircRBM23在肝细胞癌（HCC）细胞系的表达及其对HepG2细胞增殖活力及迁移能力影响。方法采用实时定量聚合酶链反应（RT-PCR）检测hsa-circRBM23在正常肝细胞系HL-7702及HCC细胞系HepG2、HHCC、HUH7和BEL-7402中的表达。转染hsa_circRBM23siRNA下调hsa_circRBM23在HCC细胞系HepG2中的表达，采用活细胞计数试剂盒（CCK-8）、EdU检测、细胞创伤愈合实验和Transwell细胞迁移实验对转染后HepG2细胞及对照组的细胞增殖活力、细胞迁移能力进行检测。结果与正常肝细胞系HL-7702比较，HCC细胞系HepG2、HHCC、HUH7和BEL-7402中的hsa_circRBM23表达量均明显升高（P〈0．01）。与对照组比较，hsa-circRBN23siRNA明显降低了hsa-circRBM23在HepG2细胞中的表达（P〈0．01）。下调hsa-circRBM23表达后，HepG2细胞的增殖活力、抑制创伤愈合、抑制细胞迁移能力下降，且HCC细胞s期细胞的百分比降低（P〈0．01）。结论Hsa_circRBM24可能在HCC发生机制中发挥一定作用，下调hsacircRBM24表达可下调HCC细胞的增殖和迁移。
Objective To investigate the expression of hsa_ circRBM23 in hepatocellular carcinoma（HCC） cell lines and explore the role of hsa_circRBM23 in the growth and migration of HepG2. Methods The expressions of hsa_circRBM23 in the normal liver cell line HL-7702 and HCC cell lines including HepG2, HHCC, HUH7 and BEL-7402 were determinated by real-time quantitative polymerase chain reaction（RT-PCR）. Hsa_circRBM23 siRNA was transfected to HepG2 cells to reduce the expression of hsa_circRBM23. Cell viability and cell migration were determinated by CCK-8 and EdU wound healing assay and Transwell cell migration assay. Results Compared with the normal liver cell line HL-7702, the expression of hsa circRBM23 was significantly up-regulated in HCC cell lines including HepG2, HHCC, HUH7 and BEL-7402 （ P 〈0. 01 ）. Hsa_circRBM23 siRNA could significantly suppressed the expression of hsa_circRBM23 in HepG2 cell line than that in the control group（ P 〈 0.01 ）. Downregulation of hsa_circRBM23 turned to significantly increase the viability of HepG2 cells, inhibit the wound closure,weaken the ability of migration and reduce the percentage of S phase cells compared with the control group（P 〈0.01 ）. Conclusion Hsa_circRBM23 may play an important role in the tumorigenic mechanism of HCC and the down-regnlation of hsa_circRBM23 may suppress the growth viability and migration of HCC cell line.
作者
王保永
张瑜
夏艳丽
肖鸿丽
赵巧飞
闫一帆
陈宏伟
Wang Baoyong;Zhang Yu;Xia Yanli(Department of gastroenterology,Luoyang Central Hospital Affiliated to Zhengzhou University,Luoyang 471000,China)
出处
《临床内科杂志》
CAS
2018年第11期777-780,共4页
Journal of Clinical Internal Medicine
