摘要
近年来，随着细胞生物学、分子生物学、组织培养技术和生物技术的发展，肝细胞的培养技术日渐成熟，体外培养肝细胞的应用也逐步得到扩展，不仅用于研究组织生理功能及其蛋白、基因表达^([1])，而旦有很多潜在的临床应用前景，如基于体外培养肝细胞的生物人工肝支持^([2])、肝细胞移植^([3])以及肝细胞介导的基因治疗^([4])等。
出处
《中国现代普通外科进展》
CAS
2000年第1期10-13,共4页
Chinese Journal of Current Advances in General Surgery
二级参考文献28
-
1李铎，中华器官移植杂志，1993年，14卷，95页
-
2Ponder KP,Gupta S,Leland F,et al.Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation. Proceedings of the National Academy of Sciences of the United States of America . 1991
-
3Kathryn D. Anderson,John A. Thompson,Judith M. DiPietro,Kate T. Montgomery,Lola M. Reid,W. French Anderson.Gene expression in implanted rat hepatocytes following retroviral-mediated gene transfer[J]. Somatic Cell and Molecular Genetics . 1989 (3)
-
4Dixit V,Arthur M,Gimick G.A morphoigical and functional evaluation of transplanted isolated encapsulated hepato-cytes following long-term transplantation in Gunn rats. Biomaterials Artificial Cells Artificial Organs . 1993
-
5Kasai S,Sawa M,Mito M.Is the biological artificial liver clinically applicable? A historic review of biological artificial liver support systems. Artificial Organs . 1994
-
6Verma I M.Gene therapy. Scientific American . 1990
-
7Andreason GL,Evans GA.Introduction and expression of DNA molecules in eukaryotic cells by electroporation. Biotechniques . 1988
-
8Wilson JM,Jefferson DM,Chowdhury JR,et al.Retrovirus-mediated transduction of adult hepatocytes. Proceedings of the National Academy of Sciences of the United States of America . 1988
-
9Chowdhury JR,Grossman M,Gupta S,et al.Long-term improvement of hypercholesterolemia after ex vivo gene therapy in LDLR-deficient rabbits. Science . 1991
-
10Friedmann T.Progress toward human gene therapy. Science . 1989
共引文献6
-
1杨继震.生物人工肝的研究现状[J].世界华人消化杂志,1999,7(3):185-187. 被引量：8
-
2李铎,孙家邦,B.Hocheletelez,RMargreiter.从手术切除肝组织和屠宰房肝组织分离肝细胞[J].中华肝胆外科杂志,1999,5(4):268-270. 被引量：5
-
3张先杰,孙家邦,李铎,孙海晨,李非.三明治构型培养肝细胞——一种潜在的生物人工肝单位[J].中华肝胆外科杂志,2001,7(5):292-294. 被引量：10
-
4江志雄.人工肝支持系统技术进展[J].实用医学杂志,2001,17(10):1009-1011.
-
5江志雄.人工肝历史、现状与展望[J].临床军医杂志,2001,29(4):91-94. 被引量：1
-
6张先杰,孙家邦,李铎,孙海晨,李非.体外培养条件下肝细胞功能研究[J].中华普通外科杂志,2001,16(12):719-721. 被引量：5
同被引文献4
-
1Bhandari RNB,Riccalton LA,Lewis AL,et al.Liver tissue engin-eering:a role for co-culture systems in modifying hepatocyte function and viability[J].Tissue Eng,2001,7(3):345-357.
-
2Mizuguchi T,Hui T,Palm K,et al.Enhanced proliferation and differentiation of rat hepatocytes cultured with bone marrow stromal cells[J].Cell Physiol,2001,189(1):106-119.
-
3Powers MJ,Domansky K,Kaazempur-Mofrad MR,et al.A mic-rofabricated array bioreactor for perfused 3D liver culture.Biotechnol Bioeng.2002,78(3):257-269.
-
4Davis MW,Vacanti JP.Toward development of an implantable tissue-engineered liver[J].Biomaterials,1996,17(3):365～372.
二级引证文献5
-
1李治国,汪艳,高毅.生物人工肝细胞材料的功能学研究进展[J].中华肝胆外科杂志,2009,15(10):795-800. 被引量：1
-
2滕丽新,刘胜学,谢庭菊,何作云.大鼠心肌微血管内皮细胞的分离培养和鉴定[J].重庆医学,2010,39(4):403-405. 被引量：6
-
3李治国,崔雪仪,汪艳,杜江,康玉占,高毅.胰腺内皮细胞共培养对C3A细胞功能变化的影响[J].中华肝胆外科杂志,2010,16(6):447-450. 被引量：1
-
4张茜,金若敏.细胞共培养技术的研究进展[J].中国药理学与毒理学杂志,2011,25(3):330-332. 被引量：19
-
5唐培培,张硕,相萍萍,黄艳,周学平.一种新型多细胞共培养皿[J].中国临床药理学杂志,2014,30(2):140-142. 被引量：1
-
1张先杰,孙家邦,宋茂民,袁辉生,段钟平,卢实春.酒精对体外肝细胞极性的影响[J].中国普外基础与临床杂志,2005,12(5):465-468. 被引量：1
-
2张先杰,孙家邦,孙海晨,李非.体外肝细胞极性的重建[J].中华普通外科杂志,2002,17(5):297-299. 被引量：5
-
3王晓英,阎明.肝细胞的体外培养及乙醛对肝细胞的形态影响[J].中国实用医药,2008,3(4):13-15. 被引量：3
-
4陈天宇,张先杰,孙家邦,孙海晨,李非.体外肝细胞极性重建与cofilin的脱磷酸化[J].中国全科医学,2007,10(11):891-893. 被引量：4
-
5张先杰,孙家邦,宋茂民,袁辉生,陈煜,段钟平.胎牛血清在体外培养肝细胞极性形成的作用[J].中华肝胆外科杂志,2005,11(9):633-635.
