摘要
目的研究酸枣仁皂苷A的抗血小板聚集活性。方法采用兔体外血小板聚集实验方法,结合酶联免疫吸附法测定血清血小板活化因子(platelet activating factors,PAF)、P-选择素和血小板因子-4(platelet factor-4,PF-4)的含量,探讨不同浓度的酸枣仁皂苷A对二磷酸腺苷(adenosine diphosphate,ADP)、花生四烯酸(arachidonic acid,AA)和PF-4诱导血小板聚集的抑制活性。结果酸枣仁皂苷A能有效抑制AA、ADP和PF-4诱导的血小板聚集,能显著降低PAF、P-选择素和PF-4的含量。结论酸枣仁皂苷A具有明显的抗血小板聚集活性。
OBJECTIVE To investigate the anti-platelet aggregation activity and mechanism of jujuboside A. METHODS The anti-platelet aggregation activity of jujuboside A was evaluated in vitro on rabbit platelet aggregation, and the content of platelet activating factors（PAF）, P-selectin and platelet factor-4（PF-4） were detected by enzyme-linked immunosorbent assay. The inhibitory effects of jujuboside A with different concentration on platelet aggregation induced by adenosine diphosphate（ADP）, arachidonic acid（AA） or PF-4 was investigated. RESULTS Jujuboside A inhibited the platelet aggregation induced by AA, ADP or PF-4, and decreased the expression levels of PAF, PF-4 and P-selectin. CONCLUSION Jujuboside A can inhibit aggregation and activity of platelet in vitro.
作者
万蒞
易蕾
王红
WAN Li;YI Lei;WANG Hong(Pharmaceutical Department,Maternal and Child Health Care Hospital of Hubei,Wuhan 430070,China;Pharmaceutical Department,Tianyou Hospital Affiliated to Wuhan University of Science and Technology,Wuhan 430064,China;School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2018年第10期1514-1516,共3页
Chinese Journal of Modern Applied Pharmacy
