摘要
目的探讨“胡椒+荜茇”挥发油化学成分及其血脑屏障通透性。方法采用水蒸气蒸馏法对胡椒、荜茇药对进行提取,得到的挥发油加入适量无水硫酸钠干燥,密闭低温保存。SD大鼠灌胃给药后取脑组织、脑脊液制备生物样品。用气相色谱-质谱联用(GC-MS)的方法,分别测定核心方剂中挥发油的成分、SD大鼠脑组织匀浆和脑脊液中的成分以及灌胃SD大鼠脑组织匀浆和脑脊液中的成分,对比分析核心方剂中透过血脑屏障的挥发油类成分。结果提取得到的药对挥发油为淡黄色透明油状液体,其中棕榈酸TMS衍生物可透过血脑屏障。结论“胡椒+荜茇”挥发油中棕榈酸TMS衍生物可透过血脑屏障,可能与脑卒中的治疗有关。
Objective To detect the volatile oil components that cross the blood-brain barrier in the treatment of stroke by pepper and fructus piperis longi.Methods Piper nigrum and Piper longum were extracted by steam distillation.The volatile oil was dried with anhydrous sodium sulfate and stored in airtight low temperature.The brain tissue and cerebrospinal fluid of SD rats were taken to prepare biological samples.The components of volatile oil in the core prescription,brain tissue homogenate and cerebrospinal fluid of SD rats,and the components of brain tissue homogenate and cerebrospinal fluid of SD rats were determined by gas chromatography-mass spectrometry(GC-MS).Results The volatile oil extracted in drug pair was a light yellow transparent oily liquid,and among them,the palmitate TMS derivative could pass through the blood-brain barrier.Conclusion The palmitate TMS derivative in volatile oil can pass through the blood-brain barrier,which may be closely related to the treatment of stroke.
作者
李婷婷
李梦媛
王雅琪
马晓玲
付雪艳
LI Tingting;LI Mengyuan;WANG Yaqi;MA Xiaoling;FU Xueyan(Ningxia Medical University,Yinchuan 750004,China;School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,China;School of Pharmacy,Ningxia Medical University,Yinchuan 750004,China)
出处
《宁夏医科大学学报》
2020年第10期1006-1012,共7页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(2018AAC03097)
宁夏高等学校一流学科建设项目(中医学)资助项目(NXYLXK2017A06)
2016年宁夏青年科技人才托举工程
宁夏医科大学2020年校级科研项目(XM2020022)。
关键词
胡椒
荜茇
挥发油
脑卒中
GC-MS
血脑屏障通透性
pepper
fructus piperis longi
volatile oil
stroke
GC-MS
blood-brain barrier permeability
