摘要
以"农大8号"和"长谷1号"2个谷子品种为试验材料,分别以3 000 r/min、5 000 r/min、7 000 r/min进行离心30 min、60 min处理,研究超重力处理对谷子种子发芽指标、萌发活力的影响以及萌发期丙二醛含量、过氧化物酶活性、超氧化物歧化酶活性的变化。结果表明,中低速离心处理能促进种子萌发、根长及鲜质量,各处理的芽长均下降;两品种各处理组的丙二醛(MDA)含量都低于对照,在处理7 000 r/min、30 min下达到最低;除了在3 000 r/min、60 min处理下长谷1号的POD活性稍有下降外,其余各处理两个品种的POD活性均高于对照,长谷1号的POD活性在5 000 r/min、30 min下达到最大值,农大8号的POD活性在处理时间60 min、转速(3 000 r/min、5 000 r/min)下达到最大;两品种的SOD活性均高于对照组,且在中高速(5 000 r/min、7 000 r/min)转速下SOD活性达到最大。超重力处理可提高谷子萌发期的抗逆性,以5 000 r/min处理时间30 min、60 min为宜。
Taking the 2 millet varieties of＂Nongda 8＂and＂Changgu 1＂as experimental materials and they were centrifuged at 3 000 r/min,5 000 r/min and 7 000 r/min for 30 min and 60 min respectively. The effects of Hypergravity treatment on germination index and germination activity of millet seeds,and the content of malondialdehyde,peroxidase and superoxide dismutase activity during the germination period were studied. The results showed that the medium-lowspeed centrifugation could promote seed germination,root length and fresh quality,and the length of shoots decreased in each treatment; The contents of malondialdehyde（MDA） in each treatment group of both varieties were lower than that of the control and reached the minimum at 7 000 r/min,30 min; In addition to the slight decrease in POD activity of Changgu 1 under 3 000 r/min,60 min treatment,the activities of POD in each treatment group of both varieties were higher than that of the control,and the POD activity of Changgu 1 reached the maximum at 5 000 r/min,30 min,the POD activity of Nongda 8 reached the maximum at a treatment time of 60 min and rotation speed of 3 000 r/min and5 000 r/min; The SOD activity of the two varieties was higher than that of the control and reached maximum at medium and high speeds（5 000 r/min and 7 000 r/min）. High-gravity treatment can improve the stress resistance of millet in the germination period,and it is appropriate to use 5 000 r/min treatment time of 30 min and 60 min.
作者
杨净
王宏富
鱼冰星
李智
王钰云
YANG Jing;WANG Hongfu;YU Bingxing;LI Zhi;WANG Yuyun(College of Agriculture in Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)
出处
《激光生物学报》
CAS
2018年第4期367-372,共6页
Acta Laser Biology Sinica
基金
山西省农业综合开发项目(201617)
山西省科技重点研发计划项目(2015-TN-09)
关键词
超重力
谷子
种子萌发指标
生理生化指标
hypergravity
millet
seed germination index
physiological and biochemical index
