摘要
本研究以大白猪、长白猪和杜洛克猪为研究对象,通过构建DNA混合池并利用PCR产物直接测序的方法,对Nramp1基因内含子6的SNP多态性进行分析。结果发现,3个猪种在Nramp1基因内含子6中均有4个SNP位点,分别为C^(111)A、C^(225)T、G^(296)T和T^(297)G。基因频率估算结果表明,等位基因C、C、G和T分别是4个SNP位点的优势等位基因。本研究结果可为猪免疫疾病研究和抗病分子育种提供理论基础。
The single nucleotide polymorphism（SNP） sites of Nramp1 intron 6 in the Yorkshire,Landrace and Duroc pigs were screened via polymerase chain reaction（PCR） and DNA sequencing anal‐ysis. The results showed that in all three pig breeds contained 4 SNP loci in the intron 6 of Nramp1 gene, which were C111A,C225T,G296T and T297G. The gene frequency estimation results showed that the alleles C, C, G and T were the dominant allele of the 4 SNP sites. The results of this study can provide a theoretical basis for swine immune disease research and disease resistance molecular breeding.
作者
付保强
彭帅
顾亚荣
赵倩
杜倩
穆晓峰
Fu Baoqiang;Peng Shuai;Gu Yarong;Zhap Qian;Du Qian;Mu Xiaofeng(College of Life Science and Engineering,Northwest Minzu University,Gansu Lanzhou 73003)
出处
《现代畜牧兽医》
2018年第10期21-24,共4页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
西北民族大学国家级大学生创新创业训练计划资助项目(项目编号:201810742134)
