摘要
【目的】探讨Nramp1基因第6内含子多态在大白猪和松辽黑猪(是由杜洛克、长白猪和民猪合成)中的分布及其与猪免疫抗病的关系。【方法】采用PCR-RFLP法对165头大白猪和109头松辽黑猪Nramp1基因第6内含子多态性与免疫功能(中性粒细胞还原力和单核细胞细胞毒百分率)的相关性进行了研究。【结果】两个品种在该位点均存在多态性,品种间、合并基因型间免疫功能差异不大,但同一品种内不同基因型间差异显著;大白猪BB基因型个体的单核细胞细胞毒百分率显著高于AB基因型个体(P<0.05),BB基因型个体的中性粒细胞NBT还原力值极显著高于AB基因型个体(P<0.01),而在松辽黑猪中则是AB基因型个体的中性粒细胞NBT还原力值显著高于BB基因型个体(P<0.05);从断奶到出栏的死亡率分析显示大白猪BB基因型个体死亡率明显低于AB基因型个体,松辽黑猪则是AB基因型个体的死亡率明显低于BB基因型个体。【结论】品种内不同Nramp1基因型与免疫功能间存在着显著相关,Nramp1基因可作为抗病力性状的一个主要候选基因。
[Objective] To study the distribution of polymorphism in natural resistance associated macrophage proteinl （Nramp 1） in two pig breeds and the association of this polymorphism with some immune functions. [ Method ] PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune function （value of polymorphonuclear leukocytes （PMN） by nitroblue tetrazolium （NBT） reduction and effect of cytotoxin of monocyte） in 165 individuals of Large White pig and 109 individuals of Songliao Black pig. [Result] There was one Nde I restriction loci in both Large White pig and Songliao Black pig. The immune traits was not significantly different in different breeds and different genotypes, but the difference was significant among different genotypes in the same breed. In Large White pig, both of values of polymorphonuclear leukocytes （PMN） by nitroblue tetrazolium （NBT） reduction and effect of cytotoxin of monocyte in genotype BB were higher than genotype AB （P〈0.05）. The death rate of genotype BB was lower than that of genotype AB. By contraries in Songliao Black pig, the value of polymorphonuclear leukocytes by nitroblue tetrazolium reduction of genotype AB was higher than that of genotype BB （P〈0.05）, and the death rate of genotype AB was lower than that of genotype BB. [ Conclusion] The results indicated that there was a significant correlation between different genotypes of Nramp1 gene and immune function in the same breed, and it could be treated as a candidate gene of disease resistance.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第1期215-220,共6页
Scientia Agricultura Sinica
基金
国家“863”项目(2003AA243030)
“十五”国家科技攻关项目(2004BA514A-13)
