摘要
目的探讨脑和急性白血病胞质(BAALC)基因的rs62527607单核苷酸多态性和儿童急性髓细胞性白血病(AML)易感性的关联。方法选取郑州儿童医院急性髓细胞性白血病(非急性早幼粒细胞白血病)患儿138例,同时选择门诊行健康体检的正常儿童166人作为对照组,两组利用连接酶检测技术检测BAALC基因的rs62527607单核苷酸多态性,同时予以AML患儿吡柔比星治疗,并记录毒副反应。结果AML组中TT、TG基因型比例高于对照组(TT:13.0%vs.2.4%,TG:43.4%vs.8.4%),GG基因型比例低于对照组(43.4%vs.89.1%),其基因分布比较差异均有统计学意义(χ^(2)=22.85,P<0.05);AML组中TT、TG基因能增加其患病风险,与对照组比较差异有统计学意义(OR=0.17,95%CI=0.05~0.49;OR=0.12,95%CI=0.06~0.23;P均<0.05)。AML女性组TT、TG基因能增加其患病风险,与对照组比较差异均无统计学意义(OR=1.07,95%CI=0.89~1.30;OR=1.25,95%CI=0.87~1.82;P均>0.05);AML男性组TT、TG基因能增加其患病风险,与对照组比较差异有统计学意义(OR=1.30,95%CI=1.09~1.51;OR=2.20,95%CI=1.12~4.31;P均<0.05);BAALC rs62527607各基因型多态性与中性粒细胞减少、血小板减少、消化道反应、肝脏毒性分级无明显相关关系(χ^(2)=4.53、3.89、5.01、5.23,P>0.05);携带等位基因G(TG+GG)有增加中性粒细胞减少、血小板减少、消化道反应、肝脏毒性的趋势,但差异无统计学意义(OR=1.16,95%CI=0.70~1.92;OR=1.15,95%CI=0.53~2.39;OR=1.72,95%CI=0.78~3.74;OR=1.23,95%CI=0.65~1.89;P均>0.05)。结论BAALC基因TT、TG基因型能增加儿童AML易感性,且有增加蒽环类药物吡柔比星毒副反应发生的趋势,但其rs62527607单核苷酸多态性与儿童AML发病风险的机制仍需进一步探讨。
Objective To investigate the association of single nucleotide polymorphism of BAALC gene rs62527607 and susceptibility of acute myelogenous leukemia(AML)in children.Methods 138 children with AML in our hospital were selected as AML group,and 166 normal children with health examination in our hospital were selected as control group;single nucleotide polymorphism of rs62527607 BAALC gene was detected in two groups by ligase detection technology.At the same time,the children with AML were given with pirarubicin purine treatment.The adverse reaction were recorded.Results The TT and TG genotype proportion in AML group were higher than that of the control group(TT:13.0%vs.2.4%,TG:43.4%vs.8.4%)and the GG genotype proportion was lower than that of the control group(43.4%vs.89.1%).The gene distribution were significant different(~5=22.85,P〈0.05).Compared with the control group,the 33?and TG gene in the AML group increased their morbid risk and the differences were statistically significant(OR=0.17,95%CI=0.05-0.49;OR=0.12,95%CI=0.06-0.23;P〈0.05).The TT and TG gene in the female AML group increased their morbid risk,but the differences were not statistically significant(OR=1.07,95%CI=0.89-1.30;OR=1.25,95%CI=0.87-1.82;P〉0.05).The T'F and TG genotypes in the male AML group increased their morbid risk and the differences were statistically significant(OR=1.30,95%CI=1.09-1.51;OR=2.20,95%CI=1.12-4.31;P〈0.05).The BAALCrs62527607 gene polymorphism was not significantly correlated with neutrophil reduce,thrombocytopenia,gastrointestinal reaction,liver toxicity classification(X2=4.53,3.89,5.01,5.23,P〉0.05).That carrying the G allele(TG+GG)increased neutropenia,thrombocytopenia,gastrointestinal reaction tendency,liver toxicity but the difference were not statistically significant(OR=l.16,95%CI=0.70~1.92;OR=l.15,95%CI=0.53-2.39;0R=1.72,95%CI=0.78~3.74;OR=1.23,95%CI=0.65~1.89;P〉0.05).Conclusion BAALC gene TT and TG genotypes could increase the susceptibility of AML in children,and may increase the adverse reaction after pirarubicin treatment,but its rs62527607 single nucleotide polymorphisms and children with AML risk mechanism still needs further discussion.
作者
史利欢
刘炜
刘俊闪
郭明发
王亚峰
SHI Li-huan;LIU Wei;LIU Jun-shan;GUO Ming-fa;WANG Ya-feng(Zhengzhou Children"s Hospital,Henan Children"s Hospital,Henan Key Laboratory of Pediatric Hematology,Zhengzhou,Henan 450000,China)
出处
《热带医学杂志》
CAS
2018年第9期1151-1155,共5页
Journal of Tropical Medicine
基金
河南省医学科技攻关计划项目(201603043)
