摘要
体外细胞毒法（四唑盐法）检测３６例急性髓系白血病（ＡＭＬ）细胞对阿糖胞苷（Ａｒａ－Ｃ）、高三尖杉酯碱（ＨＨｒ）、柔红霉素（ＤＮＲ）、阿克拉霉素（Ａｃｌａ）、长春新碱（ＶＣＲ）、足叶乙甙（ＶＰ－１６）的敏感性，同时用碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）法检测其ＣＤ＿（３４）抗原，并与临床化疗疗效比较。发现Ａｒａ－Ｃ、ＨＨｒ、ＶＰ－１６中敏组ＣＤ＿（３４）抗原的表达较高敏组高（Ｐ＜０．０５），ＤＮＲ、Ａｃｌａ、ＶＣＲ耐药组较中敏组高，差异亦显著（Ｐ＜０．０５），六种化疗药物耐药组ＣＤ＿（３４）抗原的表达均较高敏组高（Ｐ＜０．０５）。诱导化疗第一疗程后骨髓原始细胞减少指数（ＭＢＤＩ）值＜４０组，４０～６０组及＞６０组组间ＣＤ＿（３４）抗原的表达差异显著（Ｐ＜０．０５）。ＡＭＬ化疗完全缓解，部分缓解与未缓解组组间ＣＤ＿（３４）抗原表达差异亦显著（Ｐ＜０．０５）。复发患者ＣＤ＿（３４）抗原的表达较初诊者高（Ｐ＜０．０５）。ＣＤ＿（３４）阳性白血病细胞对化疗药物具有抗药性，除因其处于静止期（Ｇ＿０期）外，可能与多药耐药基因（ＭＤＲ１）的高水平表达有关。
he chemosensitivity of leukemia cells from 36 acutemyeloid leukemia (AML) patients to cytotoxic drugsincluding cytosine arabinoside (Ara-C), daunorubicin(DNR), etoposide (VP-16), vincristine(VCR),aclacinomycin (Acla)and homoharringtonine(HHr)wasdetermined by MTT assay, and the CD_(34) expression ofthe leukemia cells was detected by APAAP method.The results were compared with the elinical response tochemotherapy. It was found that the CD_(34) expressionwas significantly higher in the drug resistant groups thanin the drug-sensitive groups (P<0. 05). The significantdifference of CD_(34) expression was also found amongMBDI (myeloblast decrease index)<40 , 40～60 and>60 groups (P<0. 05) , and among complete remission ,partial remission and non-remission groups (P<0. 05).The relapsed patients showed markedly higher CD_(34)expression than that in patients at diagnosis(P<0. 05).The mechanism of the CD_(34) positive cells resistance tothe drugs might be that either they are in G_0 phase orthey have a high expression level of multidrug resistancegene (MDR1).
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第7期341-344,共4页
Chinese Journal of Hematology
关键词
药物敏感性
白血病
CD34抗原
e Chemosensitivity Acute myeloidleukemia CD_(34) antigenMultidrug resistance gene (MDR1)
