摘要
目的：基于网络药理学思路与方法,研究二妙丸中有效成分、靶点、通路之间的关系,预测其可能的＂一方多效＂药理机制。方法：依托中药分子机制的生物信息学分析工具服务器（BATMAN-TCM）对二妙丸进行成分-靶点的预测和筛选,借助检索相互作用的基因/蛋白质的搜索工具平台（STRING）构建靶蛋白互作网络,利用Bi NGO和MCODE插件对靶基因进行基因本体（GO）生物过程富集和聚类分析,通过注释、可视化和集成发现的数据库（DAVID）平台对关键靶点京都基因与基因组百科全书（KEGG）信号通路进行富集分析。结果：二妙丸活性成分作用于血清白蛋白（ALB）,丝裂原活化蛋白激酶1（MAPK1）,肿瘤坏死因子（TNF）,前列腺素内环氧合酶2（PTGS2）,5-羟色胺受体2A（HTR2A）,腺苷脱氨酶（ADA）,糖皮质激素受体（NR3C1）等37个关键靶点;参与炎症反应、神经传导、金属阳离子稳态平衡、氧化还原、脂质代谢、核苷酸代谢等GO生物过程;调控TNF,TRP通道炎症调控,FcεRI信号通路,Ⅱ型糖尿病,钙离子信号通路,5-羟色胺以及多种信号转导、肿瘤、细胞色素P450通路。结论：二妙丸除具有治疗痛风、关节炎、湿疹等炎症疾病外,还可能具有抗糖尿病周围神经病变、治疗神经系统、胃肠系统、心血管系统疾病的＂一方多效＂作用。
Objective： To study the relationship between effective ingredients,targets and pathway in Ermiaowan,and predict its possible ＂Multi-Efficacy of One Formula ＂Molecular Mechanism based on Network Pharmacology. Method： The main ingredients and targets of Ermiaowan were predicted and screened by relying on A Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine server（ BATMANTCM）. The Search Tool for the Retrieval of Interacting Genes/Proteins platform（ STRING） was used to construct target-protein interaction network,and gene ontology（ GO） bioprocess enrichment and clustering analysis of target genes were finished by using Bi NGO and MCODE plug-ins. Database for Annotation,Visualization and Integrated Discovery（ DAVID） was used to analyze kyoto Encyclopedia of Genes and Genomes（ KEGG） signaling pathways of the key targets. Result： The active ingredients of Ermiaowan acted on 37 key targets such as serum albumin（ ALB）,mitogen-activated protein kinase1（ MAPK1）,tumour necrosis factor（ TNF）, prostaglandin G/H synthase 2（ PTGS2）,5-hydroxytryptamine receptor 2 A（ HTR2 A）,adenosine deaminase（ ADA）,glucocorticoid receptor（ NR3 C1）. Got mainly involved in GO biological processes such as inflammation,nerve conduction,metal ions homeostasis, oxidation and reduction, lipid metabolism, and nucleotide metabolism; regulated signaling pathways such as TNF,TRP channel inflammatory,FcεRI,Type Ⅱ diabetes mellitus,calcium signaling pathway,serotonergic and a variety of signal transduction,tumor and cytochrome P450 enzyme. Conclusion： Besides Gout,arthritis,eczema and some inflammatory diseases,Ermiaowan may also have ＂Multi-Efficacy of One Formula＂effect on diabetic peripheral neuropathy,nervous system,gastrointestinal system and cardiovascular system diseases.
作者
刘志强
王博龙
LIU Zhi-qiang;WANG Bo-long(School of Chemical and Biological Engineering,Yichun University,Yichun 336000,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第18期198-206,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省教育厅项目(GJJ12596)
关键词
二妙丸
网络药理学
聚类分析
靶点
通路
蛋白互作
Ermiaowan
network pharmacology
cluster analysis
targets
pathways
protein-protein interaction
