摘要
以矢竹(Pseudosasa japonica)、花叶矢竹(P.japonica f.akebonosuji)和曙筋矢竹(P.japonica f.akebono)为研究对象,借助叶绿体超微结构和荧光动力学曲线的变化揭示不同叶色矢竹的光系统活性及光合特性差异。结果表明:3个竹种的光合色素含量差异明显,除花叶矢竹条纹叶白色部分叶绿体内无完整类囊体片层结构外,花叶矢竹绿条纹和曙筋矢竹的基粒数明显少于矢竹,叶绿体发育成熟度不一致;OJIP曲线及参数表明,花叶矢竹条纹绿叶和曙筋矢竹光系统II(PSII)反应中心开放降低程度低于矢竹,捕获能量用于电子传递的份额变小,PSII活性变弱;而曙筋矢竹叶片P700至初级电子受体(QA)的电子传递链氧化还原平衡偏向于还原侧,推测其光系统I(PSI)反应中心P700至PSII QA电子传递链受损。因此,PSII活性变化导致叶绿体发育不成熟,可能是引起矢竹类叶色差异的直接原因。
We explored the different photosynthetic characteristics of three Pseudosasa cultivars： P. japonica, P. japonica f. akebonosuji, and P. japonica f. akebono. The differences in photosystem activity and photosynthetic characteristics of different leaf colors were revealed by the changes of chloroplast ultrastructure and fluorescence kinetics curves. The results showed that the photosynthetic pigment content of the three species was significantly different. Except for the intact thylakoid layer structure in the white part of the chloroplast, the green streak and the radix were significantly less than the radix. The chloroplast developmental maturity is inconsistent; the OJIP curve and parameters indicate that the open reduction of the flowering green leaf and the saplings of the PSII reaction center is lower than that of the yam, the capture energy that is used for the electron transfer share to be smaller, and the PSII activity is weaker; The redox balance of the electron transport chain of bamboo leaves P700 to QA is biased towards the reducing side, and it is presumed that the P700 reaction center P700 to PSII primary electron acceptor QA electron transport chain is damaged. Therefore, the chloroplast development caused by changes in PSII activity in the photosystem is immature, which may be the direct cause of the difference in leaf color of the species.
作者
陈柯伊
李朝娜
成敏敏
赵扬辉
周明兵
杨海芸
Keyi Chen;Zhaona Li;Minmin Cheng;Yanghui Zhao;Mingbing Zhou;Haiyun Yang(State Key Laboratory of Subtropical Silviculture Zhejiang Provincial Collaborative,Innovation Center for Bamboo Resources and High-Efficiency Utilization,Zhejiang A ＆ F University,Lin＇an 311300,China)
出处
《植物学报》
CAS
CSCD
北大核心
2018年第4期509-518,共10页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.31170565)
浙江省自然科学基金(No.LY12C16002)
浙江省分析测试科技计划(No.2016C37081)
关键词
叶色变异
花叶矢竹
光合色素
叶绿体超微结构
叶绿素荧光
leaf coloration
Pseudosasa japonica f. akebonosuji
photosynthetic pigments
chloroplast ultrastructure,chlorophyll fluorescence
