摘要
目的:研究清肠温中方对2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎(UC)模型大鼠炎性因子的影响。方法:将70只雄性SD大鼠随机分为空白组,模型组,清肠温中方高、中、低剂量组,美沙拉秦组,乌梅丸组7组,除空白组外,其余大鼠均用5%TNBS/无水乙醇保留灌肠3天复制UC模型。造模后分别用蒸馏水、清肠温中方高、中、低剂量、美沙拉秦缓释颗粒和乌梅丸颗粒剂灌胃干预对应组10天,记录大鼠一般状态、疾病活动指数,酶联免疫法检测血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、干扰素γ(IFN-γ)及白介素13(IL-13)含量,免疫组化染色观察肠黏膜核转录因子κB(NF-κB)p50蛋白表达。结果:药物干预后,与空白组相比,模型组大鼠疾病活动指数明显增高(P<0.01),血清TNF-α、IL-6、IFN-γ含量明显升高(P<0.01),IL-13含量明显下降(P<0.01);肠黏膜NF-κB p50蛋白表达明显上调(P<0.01)。与模型组各项指标比较,清肠温中方中剂量和美沙拉秦组均有所改善(P<0.05)。结论:清肠温中方可以减少TNBS诱导的UC大鼠促炎因子,增加抗炎因子表达,对UC大鼠起到治疗作用。
Objective：To study the effects of intestine-clearing middle energizer-warming prescription on inflammatory factors of TNBS-induced ulcerative colitis（UC） rat model. Methods：Seventy male SD rats were randomized into seven groups, besides the blank group, other rats accepted retention enema of 5% TNBS/absolute ethyl alcohol to duplicate UC models, after modeling, the rats were intervened with distilled water, high, moderate and low doses of intestine-clearing middle energizer-warming prescription, mesalazine sustained release granules and the granules of Wu Mei pills for ten days respectively, disease activity index（DAI） and general state of the rats were recorded, the contents of IL-13, IFN-γ, IL-6 and TNF-α in the serum were detected by enzyme linked immunosorbent assay（ELISA）, and the expressions of NF-κB p50 protein in the intestinal mucosa were observed by immunohistochemical staining. Results：After drug intervention, compared with the blank group, DAI of the rats in the model group increased obviously（P〈0.01）, the contents of IFN-γ, IL-6 and TNF-α in the serum evaluated notably（P〈0.01）,the contents of IL-13 lowered remarkably（P〈0.01）; the expressions of NF-κB p50 protein in the intestinal mucosa up regulated（P〈0.01）. Compared with different indexes of the model group, these in moderate dose group of intestine-clearing middle energizer-warming prescription and mesalazine group improved（P〈0.05）. Conclusion：Intestine-clearing middle energizer-warming prescription could treat UC rats by reducing the inflammatory factors of UC rats and increasing the expressions of inflammatory factors.
作者
石磊
韩亚飞
贾博宜
郭一
丁庞华
谢添泓
孙中美
原文静
谭祥
李军祥
SHI Lei;HAN Yafei;JIA Boyi;GUO Yi;DING Panghua;XIE Tianhong;SUN Zhongmei;YUAN Wenjing;TAN Xiang;LI Junxiang(BeiJing University of Chinese Medicine,Beijing 10029,Chine;Dongfang Hospital of Beijing University of Chinese Medicine)
出处
《西部中医药》
2018年第6期16-19,共4页
Western Journal of Traditional Chinese Medicine
基金
北京市科委G20工程支撑保障项目(编号Z151100003815011)
北京中医药大学2017基本科研业务青年教师项目(编号2017-JYB-JS-111)
