摘要
以感染根结线虫的栝楼和番茄根为材料,用简化改良的贝尔曼漏斗法分离、纯化获得实验所需要的线虫;对根结线虫的体外培养进行研究,比较了灰霉菌(Botrytis cinerea)和腐皮镰孢菌(Fusarium solani)ZZG-1制备二重培养基对根结线虫生长影响,确定灰霉菌适宜作二重培养基培养根结线虫;通过接种根结线虫悬液,在室内15~25℃环境下培养3~4周,可获得大量生长一致的幼龄期(J2)线虫,解决了根结线虫培养时寄主依赖性的问题。
In this study,roots of tomato and Trichosanthes kirilowii infected by root-knot nematodes were used as experimental materials,root-knot nematodes were separated and purified by simplified and improved Bellman funnel method,and then inoculated to double medium of Botrytis cinerea and Fusarium solani separately. The results show that Botrytis cinerea was suitable for making double medium,the rootknot nematodes inoculated to double medium of Botrytis cinerea at 15-25 ℃ for 3-4 weeks,A large number of consistent juvenile stage（ J2） nematodes could be obtained,which could solve the problem of host dependence.
作者
钱骅
钱康英
陈斌
黄晓德
朱羽尧
赵伯涛
Qian Hua, Qian Kangying, Chen Bin, Huang Xiaode, Zhu Yuyao, Zhao Botao(1.Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, China; 2. College of Life Sciences, Nanjing Normal University, Nanjing 210023, Chin)
出处
《中国野生植物资源》
2018年第2期13-16,25,共5页
Chinese Wild Plant Resources
基金
江苏省政策引导类计划(产学研合作)--前瞻性联合研究项目政策引导类计划(BY2016016-01)
国家自然科学青年基金(31501462)
江苏省自然科学青年基金(BK20150067)
关键词
根结线虫
人工培养
繁殖
灰霉菌
root knot nematode
artificial culture
reproduction
Botrytis cinerea
