摘要
目的探讨血清白介素-12(IL-12)、白介素-17(IL-17)在强直性脊柱炎(AS)中的表达特征以及二者间的相关性。方法前瞻性纳入2015年2月至2017年2月收治的AS患者56例作为A组,选取同期收治的56例类风湿性关节炎(RA)患者为B组,并纳入56例健康体检者为C组。检测并比较三组IL-12、IL-17水平,利用受试者工作特征曲线(ROC)分析IL-12、IL-17对RA的预测价值。采用Pearson相关性分析明确IL-12、IL-17间的相关性,并分析二者与人类白细胞抗原B27(HLA-B27)的相关性。结果 A组、B组血清IL-12水平分别为(35.93±15.37)ng/L、(40.43±14.82)ng/L,IL-17分别为(6.63±2.83)ng/L、(10.37±3.65)ng/L,均高于C组[(23.91±5.13)ng/L、(4.59±1.16)ng/L],且B组高于A组,差异有统计学意义(P<0.05);A组稳定期IL-12、IL-17分别为(30.15±16.58)ng/L、(4.52±0.65)ng/L,均低于A组活动期[(37.93±15.12)ng/L、(5.16±0.42)ng/L],差异有统计学意义(P<0.05);IL-12预测AS的最佳截断值为37.456 ng/L,敏感度、特异度分别为80.4%、78.6%。IL-17预测的最佳截断值为8.852 ng/L,敏感度、特异度均为85.7%。通过Pearson相关性分析发现,AS患者血清IL-12与IL-17呈正相关(P<0.001),IL-12、IL-17均与HLA-B27呈正相关(P<0.001)。结论 AS患者的血清IL-12、IL-17水平较正常人明显增高,且二者与HLA-B27水平的相关性较强,可能参与了AS进展过程。
Objective To investigate the expression characteristics of serum interleukin-12（ IL-12）,interleukin-17（ IL-17） in ankylospondylitis（ AS） and the correlation between them. Methods From February 2015 to February 2017 in our hospital,56 patients with the AS were selected as group A,and during the same period 56 cases of rheumatoid arthritis（ RA） patients were selected as group B,and 56 cases of healthy check-up patients as group C. IL-12 and IL-17 levels in the three groups were detected and compared,and the predictive value of IL-12 and IL-17 on RA was analyzed by using the receiver operating curve（ ROC）. The correlation between IL-12 and IL-17 was determined by Pearson correlation analysis,and the correlation between them and human leukocyte antigen B27（ HLA-B27） was analyzed. Results The serum IL-12 in group A and group B were（ 35. 93 ± 15. 37） ng/L,（ 40. 43 ± 14. 82） ng/L,and IL-17 were（ 6. 63 ± 2. 83） ng/L,（ 10. 37 ± 3. 65） ng/L,All were higher than those in group C [（ 23. 91 ± 5. 13） ng/L,（ 4. 59 ± 1. 16） ng/L],and those in group B were higher than those in group A,and the difference was statistically significant（ P〈0. 05）. The stability of IL-12 and IL-17 in group A were respectively（ 30. 15 ± 16. 58） ng/L,（ 4. 52 ± 0. 65） ng/L,both lower than the activity period of group A [（ 37. 93 ± 15. 12） ng/L,（ 5. 16 ± 0. 42） ng/L],and the difference was statistically significant（ P〈0. 05）. IL-12 predicted that the optimal cut-off value of AS was 37. 456 ng/L,and the sensitivity and specificity were 80. 4% and 78. 6% respectively. The optimal cut-off value predicted by IL-17 was 8. 852 ng/L,and the sensitivity and specificity were 85. 7%. Pearson correlation analysis revealed positive correlation between serum IL-12 and IL-17 in AS patients（ P〈0. 001）,and IL-12 and IL-17 were positively correlated with HLA-B27（ P〈0. 001）. Conclusion The levels of IL-12 and IL-17 in patients with AS were significantly higher than that of normal people,and the correlation between them and HLA-B27 level was strong,which may be involved in the process of AS progression.
作者
解娟
归巧娣
刘文康
李博
XIE Juan;GUI Qiao-di;LIU Wen-kang(Department of Laboratory,Shaanxi Provincial People＇s Hospital,Xi＇an Shaanxi 710068,China.)
出处
《临床和实验医学杂志》
2018年第15期1621-1623,共3页
Journal of Clinical and Experimental Medicine
