摘要
目的探讨缺氧缺血性脑损伤(HIBD)新生鼠miR-210表达与脑血管再生的关系。方法采用Rice-Vannucci法制备HIBD新生鼠模型,按照模型制作类型分为模型组、假手术组和对照组,分别于模型制备后第1、3、7天取脑组织标本,采用HE染色观察脑组织病理变化,免疫组织化学染色法检测脑组织血管内皮生长因子(VEGF)和CD34蛋白表达水平,并计数微血管密度(MVD),实时定量荧光PCR检测脑组织miR-210表达水平。结果对照组和假手术组大脑组织结构清晰,模型组术后神经元细胞出现凋亡和固缩核。对照组、假手术组小鼠脑组织miR-210表达、VEGF表达、MVD计数在HIBD后第1、3、7天间比较差异无统计学意义(均P>0.05)。与HIBD后第1天比较,模型组HIBD后第3、7天miR-210表达、VEGF表达、MVD计数均上升,差异具有统计学意义(均P<0.05);HIBD后第1、3、7天模型组miR-210表达、VEGF表达、MVD计数明显高于对照组和假手术组,差异具有统计学意义(均P<0.05)。相关分析显示,HIBD小鼠脑组织miR-210表达与MVD、VEGF呈明显正相关关系(r=0.645,0.710,均P<0.05)。结论缺氧缺血脑损伤后可诱导miR-210表达上调,促进脑组织VEGF表达和血管再生。
Objective To explore the the relationship between miR-210 expression and angiogenesis hypoxic-ischemic brain injury in neonatal mice.Methods A model of hypoxic-ischemic brain injury in neonatal in mice was established by Rice-Vannucci method,Mice were randomly divided into model group,sham group and control group according to the model establishment type.The brain tissues were collected 1,3,and 7 days after operation,the histopathologic changes were observed by hematoxylin and eosin（HE）staining.The protein levels of VEGF and CD34 were measured by immunohistochemical staining,and microvascular density（MVD）was determined.The expression levels of miR-210 were performed by real-time PCR.Results The structure of brain tissue was clear in the control group and sham group,while neuronal apoptosis and pyknosis were observed in the model group.The miR-210 expression,VEGF level and MVD count 1,3,7 day（s）after operation were not significantly different between the control group and the sham group（all P0.05）.The miR-210 expression,VEGF level and MVD count was significantly increased in the model group 3 and 7 days after HIBD establishing,when compared with the results 1 day after HIBD establishing,and the differences were statistically significant（all P0.05）.Additionally,these indexes in the model group were significantly higher than those in the control group and sham group 1,3 and 7 days after operation（all P0.05）.Correlation analysis showed there was a significant positive correlation between miR-210 and MVD,VEGF（r=0.645,0.710,all P0.05）.Conclusion Hypoxia-ischemia brain damage can induce the expression of miR-210,and up-regulation of VEGF and angiogenesis in HIBD mice.
作者
梁国安
姚桂飞
范海玲
Liang Guoan;Yao Guifei;Fan Hailing(Department of Paediatrics;Department of Respiratory Medicine Taizhou Hospital of Enze Medical Center,Taizhou 317000)
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2018年第4期431-436,共6页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
浙江省台州市科技计划A类项目(No.15yw01)
浙江省台州恩泽医疗中心(集团)科学研究基金资助项目(No.14EZC14)
