摘要
研究利用黑曲霉发酵强化对草石蚕的提取,运用高效液相色谱-示差折光检测法(HPLC-RID)对得到的水苏糖进行分析。采用单因素实验方法,探究了提取工艺中是否带渣发酵、蔗糖酶抑制剂、发酵培养方式等因素对水苏糖浓度的影响;确定提取水苏糖的最佳工艺条件。结果表明:接种量为提取液重量0.02%的黑曲霉,添加提取液重量0.01%的蔗糖酶抑制剂乙二胺四乙酸二钠(EDTA-2Na),采用静置-液体震荡两阶段培养方式进行带渣发酵培养,发酵温度为30.0℃,时间为24.0h,水苏糖纯度可达到80.43%。
The effect of extraction and purification of stachyose from Stachys seiboibi has been strengthened by fermentation method with Aspergillus niger.The obtained stachyose is analysed using HPLC-RID method.The single factor experiments are taken to study the effects of fermentation with dregs or not,invertase inhibitors,fermentation methods on the concentration of stachyose,determine the optimum conditions for the extraction of stachyose.The results show that the inoculum size is0.02% by weight of Aspergillus niger and 0.01%by weight of sucrase inhibitor ethylenediamine tetraacetic acid disodium（EDTA-2 Na）,the culture of slag fermentation is carried out by means of static-liquid shock,the fermentation temperature is 30.0 ℃,the fermentation time is 24.0 h,and the stachyose purity reaches 80.43%.
作者
周文斯
熊犍
谢瑾
崔春
王炜
ZHOU Wen-si;XIONG .lian;XIE Jin;CUI Chun;WANG Wei(College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China)
出处
《中国调味品》
CAS
北大核心
2018年第7期21-25,32,共6页
China Condiment
基金
国家自然科学基金项目(31201416
31571821)
广州市科技计划项目(201604020067)
关键词
草石蚕
水苏糖
黑曲霉
发酵
纯化
Stachys seiboibi
stachyose
Aspergillus niger
fermentation
purification
