摘要
采用EPR波谱仪测定肺组织内自由基变化以评价快速减压对肺的损伤。把SD大鼠在0．5MPa空气中暴露60min，用1min匀速减至常压，然后分别在45min和90min时腹腔放血处死（处死前25min注入0．05M的PBN，0.5ml／100g），取肺0.75g匀浆后用EPR波谱仪测定维生素C自由基（Vc）强度。结果显示减压后45min肺组织内Vc强度无明显变化，减压90min组的肺组织内Vc强度明显增高（P＜0．05）。表明快速减压可引起肺组织自由基增加。
The purpose of this experirment was to evaluate decompression-induced pulmonary injury by means of measurement of ascorbate free radical (Vc) alteration in pulmonary tissue of the rats which experienced rapid decompression. 29 male S-D rats were divided into 4 groups: A was executed at 45 min post-decompression; B was control for A; C was executed at 90 min;D was ontrol for C. Group A and group C were to compress up to 0. 5 MPa(air) and stay for 60 min, then were decompressed smoothly to normbaric in 1 min. The 0.05M N-tert-butylalpha-phenylnitrone (PBN) was given at 20 min after completion of decompression (groups A and B) or at 65 min (groups C and D). At 25 min after PBN injection the animals were killed by cutting arteria coeliaca, and 0. 75 g pulmonary tissues were taken. These tissues were homogenized for electron paramagnetic resonance (EPR) assay.The results demonstrated that the ascorbate free radical (Vc) signals were obtained at all samples. The intensity of signals measured in group C rose markedly (P＜0.05) and that in group A was close to control's. So it can be concluded that rapid decompression would increase the generation of free radical in lung.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2000年第2期238-240,共3页
Journal of Biomedical Engineering
