摘要
目的探讨不同提取方法对野棉花抗炎镇痛活性的影响。方法将野棉花全草制备成不同提取物。采用扭体与电热板法观察其镇痛作用,应用小鼠耳廓肿胀、足趾肿胀及棉球肉芽肿胀实验综合评价其抗炎活性。结果与空白对照组比较,小鼠耳廓肿胀、足趾肿胀及棉球肉芽肿胀实验水部位低剂量的抗炎作用差异无统计学意义(P>0.05),其余各剂量提取部位均有显著的抗炎镇痛作用(P<0.05)。乙酸乙酯部位在热板法镇痛试验中活性最强。正丁醇部位在小鼠耳廓肿胀、足趾肿胀及棉球肉芽肿胀抗炎实验中和扭体法中活性最强。结论野棉花全草具有明显的抗炎镇痛作用,正丁醇部位与乙酸乙酯是主要有效部位。
Objective To investigate the effect of different extraction methods on anti-inflammatory and analgesic activity of Anemone hupehensis. Methods The different abstracts were prepared from the whole herb of Anemone hupehensis.The analgesic effect was observed by adopting the mouse torsion and electric heating plate method,and the anti-inflammatory activity was comprehensively evaluated by using the mouse ear tumefaction,toe tumefaction and tampon granulation tumefaction exprements. Results Compared with the blank model group,the anti-inflammatory action difference of low dose in the water layer parts of mouse ear tumefaction,toe tumefaction and tampon granulation tumefaction had no statistical significance（ P 〉0.05）,and the extracting parts of rest doses all had significant anti-inflammatory and analgesic effect （ P 〈0.05）.Ethyl acetate part had strongest activity in the electric heating plate experiment.N-butanol part had strongest activity in the ear tumefaction,toe tumefaction,tampon granulation tumefaction experiments and torsion method. Conclusion The whole herb of Anemone hupehensis has prominent anti-inflammatory and analgesic effect,and the ethyl acetate part E and N-butanol part are main effective parts.
作者
李香
许义红
孙晓惠
韩忠耀
付云燕
伍俊科
LI Xiang;XU Yihong;SUN Xiaohui;HAN Zhongyao;FU Yunyan;WU Junke(Qiannan Medical College for Nationalities,Duyun,Guizhou 558000,Chin)
出处
《重庆医学》
CAS
2018年第15期1990-1992,1996,共4页
Chongqing medicine
基金
黔南医专科研基金资助项目(QNYZ201413)
关键词
野棉花
抗炎
镇痛
活性部位
Anemone hupehensis Lem
anti-inflammatory
analgesia
active site
