摘要
【目的】分析湛江湾沉积物中氨氧化古菌(AOA)和氨氧化细菌(AOB)的丰度与多样性。【方法】采用分子生态学方法。【结果】AOA amoA基因丰度范围为3.23×10~5~5.27×10~6 copies·g^(-1)(以干土计),AOB amoA基因丰度范围为2.99×10~4~1.06×10~7 copies·g^(-1)(以干土计),两者平均丰度差别不大;但对于潮下带,大部分站位AOA amoA基因丰度高于AOB,且与氨氮和有机碳含量显著正相关。在工厂排污口和养殖区AOA多样性高于其他站位,而AOB则相反,说明AOA对环境污染物有更强的适应能力。系统发育分析显示,88%的AOA amoA序列属于海洋簇Group I.1a,优势类群是一类喜热带气候的AOA新分支;潮下带亚硝化螺菌属AOB为优势种群,而潮间带亚硝化单胞菌属AOB为优势种群。CCA分析表明,盐度和pH显著影响湛江湾AOA与AOB的群落结构。【结论】湛江湾沉积物中氨氧化微生物的分布与氨氮、总有机碳、盐度、pH等多种环境因子密切相关。
【Objective】To analyze the abundance and biodiversity of ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） from sediments of Zhanjiang Bay. 【Method】 Molecular ecological approach was used. 【Results】The number of AOA amoA ranged from 3.23 × 10^5 to 5.27 × 10^6 copies·g^-1（in dry deposit soil, the same below） while that of AOB amoA ranged from 2.99×10^4 to 1.06×10^7 copies·g^-1. Their average abundance was almost equal. However, when only the subtidal zone was considered, the abundance of AOA amoA gene was higher than that of AOB amoA gene for most of sites. In combination with significant positive correlation between AOA amoA gene and ammonium concentration or total organic carbon, we speculated that AOA dominated the ammonia oxidation in subtidal zone of Zhanjiang Bay. The diversity of AOA in sites close to sewage outfalls of plants and aquaculture zone was higher that other sites, whereas the trend of AOB was opposite. It suggest that AOA had higher adaptive capacity for environments with pollutants than AOB. Phylogenetic analysis found that 88% of AOA amoA sequences were affiliated with Group I.1 a of marine AOA cluster, and the dominants were a new clade of AOA. In subtidal zone, Nitrosospira was the dominant AOB while Nitrosomonas was the dominant AOB in intertidal zone. CCA analysis showed that community structures of AOA and AOB was significantly influenced by salinity and pH. 【Conclusion】Distribution patterns of ammonia-oxidizing microorganisms in sediments of Zhanjiang Bay was closely correlated with several environmental factors including ammonium, total organic carbon, salinity and pH.
作者
阳雯娜
董宏坡
侯庆华
李雁群
陈法锦
周欣
毛铁墙
YANG Wen-na;DONG Hong-po;HOU Qing-hua;LI Yan-qun;CHEN Fa-jin;ZHOU Xin;MAO Tie-qiang(College of Food Science and Technology, Guangdong Ocean University;College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, China)
出处
《广东海洋大学学报》
CAS
2018年第2期37-46,共10页
Journal of Guangdong Ocean University
基金
广东省自然科学基金(2016A030312004
2015A030313616
2015A030313326)
国家自然科学基金(41576123)
广东省科技计划项目(2017A050501038)
湛江市科技项目(2015A03026)
关键词
氨氧化微生物
基因
AMOA
丰度
多样性
分布
沉积物
湛江湾
ammonia-oxidizing microorganism
gene
amoA
abundance
diversity
distribution
sediments
Zhanjiang Bay
