摘要
从浙江舟山桃花岛近海海域分离筛选到一株高活性氨氧化细菌,命名为THD-1。菌株THD-1为革兰氏阴性,球状至椭球状,大小约为(0.5～0.8)μm×(0.7～1.3)μm;菌落呈无色透明状,针尖大小,边缘光滑;可在盐度为0～50‰的培养基中生长。16S rDNA序列比对表明,菌株THD-1与Nitrosomonas europaea ATCC25978T的相似性为96.3%。培养液的pH值对菌体生长和氨氧化活性影响明显,当pH值降至6.0以下时,菌株的生长和氨氧化活性几乎完全被抑制,但这种抑制可通过回调pH值的方法解除;在所试的4种碱液中,碳酸氢铵调节pH值对菌体生长和氨活性改善效果最好。建立了菌株THD-1的分批补料式高密度培养方法,最终OD600可达0.214,最大活菌数可达7.5×108 cells/mL。
A strain of ammonia-oxidizing bacteria, THD-1, was isolated from the coastal surface sediment of Taohua Island in Zhoushan. Its cell is spherical or short rod with a size of 0. 5 - 0. 8 μm×0. 7 - 1.3 μm, gram-negative, and the colony is colorless and transparent, needle size with a smooth edge. The similarity between the 16S rDNA sequence of the srain THD-1 and that of Nitro- somonas europaea ATCC25978^T is 96. 3%. The pH of culture solution significantly affects cell growth and ammonia oxidation of strain THD-1. Ammonia bicarbonate could improve the growth and ammonia oxidation activity when used for pH controlling. High-density culture for strains THD-1 was obtained by fed-batch adding of ammonia bicarbonate, with a maximum of 0. 214 OD600 and 7.5 × 10^8 cells/mL.
出处
《生物学杂志》
CAS
CSCD
2012年第5期81-84,共4页
Journal of Biology
基金
教育部科学技术研究重点项目(208053)
宁波市科技计划项目(2008C50027)
关键词
氨氧化
亚硝化单胞菌
分批补料
高浓度培养
ammonia-oxidizing
Nitrosomonas
fed-batch
high-density cell culture
