摘要
人骨髓冻存后其造血祖细胞活力有一定程度下降,本研究对这种下降的可逆性作了初步观察。结果发现,用双层法和单层法作CFU-GM培养时,未冻存骨髓集落产率相近,冻存骨髓双层法的CFU-GM产率高于单层法。骨髓细胞用20％FM-CM、PHA-LYCM、PHA-PMCM预孵育2h后,分别测定其CFU-GM、BFU-E与CFU-Mix,发现这种孵育过程对未冻存骨髓的集落产率无明显影响,而冻存骨髓的集落产率在孵育后可升高(GEMmeg除外)。说明骨髓造血祖细胞对冻存的损伤反应不均一,部分受损细胞在一定条件下可以恢复其增殖活力。这对于用冻存骨髓作骨髓移植可能有一定意义。
The reversibility of decreased viability of hemopoietic cells in human bone marrow after cryopreservation was studied. It was found that the CFU-GM yields of non-cryopreserved bone marrow cells culured by bilayer method were similar to that cultured by monolayer method. For cryopreserved sample, the CFU-GM yield of bilayer method was higher than that of monolayer method. Preincubation with 20% FM-CM, PHA-LYCM or PHA-PMCM for 2 hours had no effects on the yields of CFU-GM, BFU-E and CPU-Mix for non-cryopreserved bone marrow cells, while for cryopreserved bone marrow, the yields incresed after preincubation (except that of CFU-GEMmeg). It is suggested that the injurious effect of cryopreservation on bone marrow hemopoietic cells is nonuniform. Under certain conditions, the declined viability of a portion of injured progenitor cells could be reversed to normal. It seems likely that this finding is meaningful for bone marrow transplantation with cryopreserved bone marrow.
出处
《中国应用生理学杂志》
CAS
CSCD
1991年第4期328-331,共4页
Chinese Journal of Applied Physiology
关键词
造血细胞
冷冻损伤
骨髓冻存
viability of hemopoietic cells
cryo-injury
cryopreserved bone marrow
