摘要
本研究分析了超低温冻存前后外周血淋巴细胞(LC)的转化活性改变.其结果发现,冻存后T淋巴细胞(TLC)对植物凝集素(PHA)刺激反应迟缓,转化率减低(冻前72小时为66.19%,冻后72小时为48.93%),延长培养时间(96小时)可使部分细胞功能得以恢复,但仍不能达到冻前水平(冻前65.69%,冻后58.2%),冻存后TLC转化中的母细胞型和分裂型百分比率下降明显,前者由38.23%减至23.48%,后者由1.29%减至0.36%,说明超低温冻存可影响TL内蛋白质和核酸合成,对B淋巴细胞(BLC)影响不明显.
The transformation activities of pre-and post-cryopreservation of peripheral lymphocytes were analysed. It was found that after cryopreservation the reactivity of TLC to PHA was delayed and their transformation rate was reduced (86.19% of unfrozen cells vs 48. 93% of frozen cell). Prolongation of the duration of cultivation (96h) can restore the function of a part of frozen cells (at 96th h, 65. 69% of unfrozen cells vs 58. 2% of frozen ones). With the assay of TLC transformation),it was found that the portions of blastic type and mitotic type decreasea significantly (from 38. 23% to 23. 48% for the former,from 1. 29% to 0. 36% for the later,respectively). It was suggested that under cryopreservation,the process of transformation was inhibited by the disturbance of the synthesis of protein and nucleic acid on TLC. BLC had no change obviously.
出处
《海军总医院学报》
1996年第1期17-19,共3页
Journal of Naval General Hospital of PLA
