摘要
本研究利用热处理和Sephadex G-150凝胶过滤层析等方法,从原发性高血压病患者(EHS)红细胞中部分纯化了抗高血压因子(AHF)。AHF具有热稳定性,分子量大于6kDa;能明显降低卒中易感型自发性高血压大鼠(SHR_(sp))血压,腹腔一次注入AHF(1.6mg/kg)30min后,SHR_(sp)收缩压从原来的27.6±0.7kPa降低到21.4±0.8kPa(p<0.001),4h后收缩压恢复至原水平。AHF能显著抑制自发性高血压大鼠(SHR)和肾性高血压大鼠(RHR)主动脉(A)及肠系膜动脉(MA)血管平滑肌(VSM)Ca^(2+)内流。且对MA Ca^(2+)内流的抑制作用强于A。以上结果表明:EHS红细胞中存在AHF,能显著降低高血压大鼠血压,其降压机制可能与其抑制VSM特别是小动脉VSM Ca^(2+)内流有关。
Utilizing heat treatment and gel filtration chromatography on Sephadex G-150, antihypertensive factor (AHF) was partially purified from erythrocytes of essential hypertensive subjects. AHF is heat-stable and its molecular weight is greater than 6 000 dalton. AHF was administered by single intraperitoneal injection to stroke prone spontaneously hypertensive rats (SHRsp) and Wistar Kyoto (WKY) rats. 30 minutes after injection, the systolic blood pressure (SBP) in SHRspwas lowered significantly (27.6 ± 0.7 to 21.4± 0.8kPa) and recovered to near original level 4 hours after administration of AHF. AHF could significantly inhibit calcium uptake in vitro either by aortic strips(A) or by mesenteric artrial segments (MA) from both spontaneously hypertensive rats (SHR) and renal hypertensive rats (RHR), and the inhibitory effects were greater in MA than in A.The results indicated that AHF was present in erythrocytes of essential hypertensive patients and significantly decreased the SBP in SHRsp. The mechanism of depressor effect of AHF may be related to the inhibitory effects on calcium uptake of vascular smooth muscle, especially of resistance vessel smooth muscle.
出处
《中国应用生理学杂志》
CAS
CSCD
1991年第3期198-202,共5页
Chinese Journal of Applied Physiology
关键词
高血压
红细胞
抗高血压因子
essential hypertension
erythrocyte
antihypertensive factor
calcium uptake.
