摘要
建立了在同一血样中测定茶碱和咖啡因的高效液相色谱法。具有简便、快速、准确、可靠、灵敏度高、专一性好等优点。二药的检测下限均为0.02μg。平均回收率均为100.9%。在4～32μg/ml的线性范围内,茶碱和咖啡因的相关系数为γ=0.9999和γ=0.9983。血清用量只需25μl,尤适用于临床哮喘病人和早产儿、新生儿支气管发育不良的常规监测和临床药理的研究。
The paper had established a method for the determination of theophyllineand caffeine in a same blood sample by HPLC. Theirdetection limit was100.9%, The linear relative coefficients of theophylline and caffeine were r=0.9999and r=0.9983 respectively, between the range from 4 to 32μg/ml. only 25μl serumwas needed for this method. This method was simple, rapid, accurete, reliable,sensitive and had good specificity, Therefore. it is especially applicable for theroutine monitor of the patient with asthma and premature andnewborn infantwith badly developed bronchus and the researeh of clinical pharmacology.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1991年第12期532-535,共4页
Chinese Journal of Hospital Pharmacy
关键词
茶碱
咖啡因
血药浓度
HPLC
Theophylline
Caffeine
HPLC
Plasma Concentration
