摘要
过氧化氢酶(catalase,CAT)是生物防御体系的关键酶之一,可促进体内的H_2O_2分解为H_2O和O_2。过氧化氢酶由CAT基因编码,目前已在一系列物种中得到克隆。黄瓜是葫芦科重要蔬菜作物,对CAT酶的功能还知之甚少。为了探讨黄瓜过氧化氢酶的功能,本实验以华北型黄瓜9930为实验材料,应用荧光定量PCR方法分析了黄瓜CsCAT3基因在NaCl、高温和低温等逆境胁迫下表达量的变化;并构建该基因的过量表达载体,用农杆菌介导将CsCAT3基因转化拟南芥,对转基因拟南芥植株进行了耐盐能力分析。结果表明:(1)在NaCl、高温和低温胁迫下CsCAT3基因被诱导增强表达,在NaCl和高温处理6 h后,表达量分别为对照的11.72倍和10.84倍;在冷处理6 h后,其表达量为对照的6.8倍;其中以200 Na Cl增强表达最为明显。(2)过量表达CsCAT3基因的拟南芥转基因植株在200 mmol/L NaCl处理条件下,其耐盐能力明显增强。
CAT（catalase） is one of the key enzymes in biological defense system, which can promote the decomposition of H2O2 into H2O and O2 in vivo. Catalase is encoded by CAT gene, and it has been cloned in a series of species. Cucumber is an important vegetable crop in the gourd family, but little is known about the function of CAT enzyme gene. In order to investigate the function of catalase in cucumber, the cucumber of North China type 9930 was used as experimental material in this experiment. The expression of cucumber CsCAT3 gene under NaCl, high temperature and low temperature stress was analyzed by using fluorescent quantitative PCR method.Also, the overexpression vector of CsCAT3 gene was established, and the CsCAT3 gene was transformed into Arabidopsis by Agrobacterium transformation. The ability of salt tolerance of transgenic Arabidopsis plants was analyzed. The results showed：（1） Under NaCl, high temperature and low temperature stresses, CsCAT3 gene was induced to enhance expression. After NaCl and high temperature treatment for 6 h, the expression levels were11.72 times and 10.84 times higher than those of the contrast; after cold treatment for 6 h, the expression level was6.8 times higher than that of the contrast; among them, the 200 NaCl enhanced expression was the most obvious;（2） Under the condition of 200 mmol/L NaCl treatment, the salt tolerance of the Arabidopsis transgenic plants ofoverexpression CsCAT3 gene was significantly enhanced.
作者
杨子建
周勇
戈伶俐
李光华
刘强
徐雅萍
蒋伦伟
杨寅桂
YangZijian 1, Zhou Yong2 ,Ge Lingli 1, Li Guanghua2, Liu Qiang1, Xu Yaping 1, JiangLunwei 2, YangYingui1(1. School of Agriculture, Jiangxi Agricultural University, Nanchang, 330045; 2 School of Sciences, Jiangxi Agricultural University, Nanchang, 330045)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第3期807-812,共6页
Molecular Plant Breeding
基金
江西省研究生创新专项资金项目(YC2016-S189)
江西现代农业科研协同创新专项(JXXTCX2015005002)共同资助
关键词
过氧化氢酶
CsCAT3
抗逆
拟南芥
转化
Catalase, Cs CA T3, Stress resistance, A rab idopsis thaliona, Transformation
