摘要
目的：探讨参附注射液治疗重症急性胰腺炎模型大鼠心肌的保护作用。方法：选取雄性SD大鼠60只，随机分为6组，分别为假手术组、模型组、乌司他丁组（2×10^4U·kg^-1）和参附注射液高、中、低剂量组（3，1．5，0．75g·kg^-1）。实验采用逆行胆胰管灌注5％牛磺胆酸钠溶液复制重症急性胰腺炎大鼠模型，造模后3h，分别给予尾静脉注射生理盐水、乌司他丁、不同剂量参附注射液。6h后苏木素-伊红（HE）染色法观察各组大鼠胰腺及心肌的病理变化，并检测大鼠心肌细胞Na＋-K＋-ATP酶活性，实时荧光定量聚合酶链式反应（qPCR）法检测肿瘤坏死因子-α（TNF-α），白细胞介素-1β（IL-1β）和白细胞介素·6（IL-6）mRNA表达量。结果：与假手术组比较，模型组的大鼠胰腺、心肌损伤严重，病理评分明显升高（P〈0．05），心肌Na＋-K＋-ATP酶活性明显降低（P〈0．05），TNF．d，IL-1／3mRNA表达量明显升高（P〈0．05）；与模型组比较，乌司他丁组、参附注射液组的胰腺和心脏病理评分明显降低（P〈0．05），参附注射液组大鼠心肌Na＋-K＋-ATP酶活性明显增加（P〈0．05），TNF-α，IL-lβmRNA表达量明显降低（P〈0．05）。结论：参附注射液可能通过改善心肌细胞Na＋-K＋-ATP酶活性，减少心肌组织中TNF-α，IL-1β的表达，对心肌起保护作用。
Objective： To investigate the protective effect and mechanism of Shenfu injection on myocardium in rats with severe acute pancreatitis. Method： Totally 60 male SD rats were randomly divided into 6 groups： Sham operation group, model group, ulinastatin （2 ×10^4 U.kg^-1） group and Shenfu injection groups （3, 1.5, 0.75 g.kg-l）. The models of severe acute pancreatitis were established by retrograde cholangiopancreatic injection of 5% sodium taurocholate solution. Three hours after modeling, the normal saline, ulinastatin and Shenfu injection were respectively given by tail vein injection. After 6 hours, the pathological changes of pancreas and myocardium were observed; the expression of Na ＋-K＋-ATPase activity was detected; and the mRNA expression levels of tumor necrosis factor-or （TNF-α） , interleukin-lβ （IL-lβ） and interleukin-6 （IL-6） in rat cardiomyocytes were detected by Real-time fluorescent quantitative polymerase chain reaction （qPCR）. Result： As compared with the sham operation group, the pancreatic and myocardial injuries were severe and pathological score was significantly increased in model group （/9 〈 0.05 ） ; the activity of Na ＋ -K -ATPase was significantly decreased （P 〈 0.05） , and the mRNA expression levels of TNF-α and IL-1β were significantly increased （P 〈 0.05 ）. As compared with the model group, the pancreatic and cardiac pathological scores were significantly decreased in Ulinastatin group and Shenfu injection groups （P 〈 0.05） ; the activity of Na ＋ -K ＋ -ATPase in the myocardium was significantly increased and the mRNA expression levels of TNF-α and IL-βwere significantly decreased in Shenfu injection group （P 〈 0.05 ）. Conclusion： Shenfu injection may improve the activity of Na＋-K-ATPase in myocardial cells and decrease the mRNA expression of TNF-αand IL-β in myocardium to protect the myocardium.
作者
杨伟钦
李培武
米红
赵迪克
向长勤
陈昫
刘凤斌
YANG Wei-qin1, LI Pei-wu2. , MI Hong2 , ZHAO Di-ke1 , XIANG Chang-qin1, CHEN Xu2, LIU Feng-bin2.(1. Guangzhou University of Traditional Chinese Medicine （TCM）, Guangzhou 510405, China; 2. First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第7期159-164,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省省级科技计划项目(粤科规财字[2015]72号2014A020209071)
广州中医药大学第一附属医院创新强院工程项目(岭南中医邓氏内科流派,2015LP01)
广东省中医药局建设中医药强省专项资金中医优势病种突破项目(粤中医函[2015]19号)
