摘要
目的采用循环增强荧光免疫法和电化学发光法定量检测降钙素原(PCT),对其检测结果的一致性进行比较分析。方法以电化学发光法为对比方法,循环增强荧光免疫法为实验方法,用两种方法分别检测219例住院患者的样本。循环增强荧光免疫法检测的血清组(Core血清组)结果、微量全血组(Core微量全血组)结果与电化学方法检测的血清组(Roche血清组)结果进行两两进行配对t检验和相关性分析,计算在医学决定水平(0.5ng/mL与2.0ng/mL)的相对灵敏度、相对特异性、约登指数并进行Kappa一致性检验。结果Core血清组、Core微量全血组与Roche血清组三者之间比较均无统计学差异(P>0.05)。Core血清组与Roche血清组呈正相关(r=0.993,P<0.01),一元直线回归方程为Y=-0.061+1.041 X(0.04≤X≤60)。Core微量全血组与Roche血清组成正相关(r=0.989,P<0.01),一元直线回归方程为Y=0.022+1.023 X(0.04≤X≤60)。Core血清组和Core微量全血组呈正相关(r=0.986,P<0.01),一元直线回归方程为Y=0.129+0.973 X(0.04≤X≤60)。三个对比组在医学决定水平(0.5ng/mL和2.0ng/mL)的相对灵敏度均大于92%,相对特异性均大于95%,约登指数与Kappa值均大于0.9,表明一致性较好。结论循环增强荧光免疫法与电化学发光法检测结果具有很好的一致性,符合临床检测要求。
Objective To use cyclic amplified fluorescence immunoassay method and electrochemiluminescence immunoassay method for quantitative detection of procalcitonin（PCT）,and to evaluate the consistency of the test results.Methods With the method of electrochemiluminescence used as the contrast method and the cyclic enhanced fluorescence immunoassay method used as experimental method,samples of 219 hospitalized patients were measured in two ways.The results were divided into three groups：the serum group（Core serum group）and the micro blood group（Core micro blood group）which were detected in cyclic amplified fluorescence immunoassay method and the serum group（Roche serum group）which was detected by electrochemiluminescence immunoassay method.The data of three groups were compared with each other in paired t test,and correlation analysis,the relative sensitivity,the relative specificity,and Jorden index at the medical decision level（0.5 ng/mL and 2.0 ng/mL）were calculated and the Kappa Consistency Test was calculated.Results There were no statistical differences in three groups（〉P0.05）.The Core serum group was positively correlated with the Roche serum group（r=0.993,P〈0.01）.The linear regression equation was Y =-0.061＋1.041 X（0.04≤X≤60）.The Core micro blood group was positively correlated with the Roche serum group（r=0.989,P〈0.01）.The linear regression equation was Y=0.022＋1.023 X（0.04≤X≤60）.The Core micro blood group was positively correlated with the Core serum group（r=0.986,P〈0.01）.The linear regression equation was Y=0.129＋0.973 X（0.04≤X≤60）.The relative sensitivity of the three comparison groups was greater than 92% and the relative specificity was greater than 95% at the medical decision level（0.5 ng/mL and 2.0 ng/mL）,and the Jorden index and Kappa values were greater than 0.9.It indicated a better consistency.Conclusion Cyclic amplified fluorescence immunoassay and electrochemiluminescence immunoassay detection results were very consistent,which meet the clinical testing requirements.
出处
《国际检验医学杂志》
CAS
2018年第5期588-590,594,共4页
International Journal of Laboratory Medicine
关键词
降钙素原
循环增强荧光免疫法
电化学发光法
procalcitonin
cyclic amplified fluorescence immunoassay method
electrochemiluminescence immunoassay method
