摘要
目的 :探讨维拉帕米对α1( )胶原基因启动子活性的影响。方法 :将人α1( )胶原基因启动子重组体 p COL H 1.5、p COL H2 .5转染至大鼠肾小球系膜细胞 ,分别经不同浓度的转化生长因子β1(TGF-β1)和维拉帕米处理后 ,EL ISA法测定细胞 CAT表达水平。结果 :TGF- β1作用后 ,p COL H1.5和 p COL H2 .5的 CAT活性明显增加 ,分别为对照组的 1.4 98和 1.5 5 1倍 ;维拉帕米对两个重组体的 CAT表达均有抑制作用 ,与单纯 TGF- β1组比较 ,维拉帕米加 TGF- β1组 p COL H2 .5的 CAT活性明显降低 (维拉帕米低浓度组 P<0 .0 5 ,高浓度组 P<0 .0 1)。结论 :维拉帕米不仅直接抑制启动子的活性 ,而且还部分阻断 TGF- β1诱导的 α1( )胶原基因启动子激活。
Objective:To explore the effect of verapamil on the activition of α1(Ⅰ) collagen gene promotor induced by TGF β1. Methods: The recombinant pCOLH 1.5 and pCOLH2.5 of α1(Ⅰ) collagen gene promotor were transfected to rat mesangial cell and were treated with different concentration of TGF β1 and verapamil. The activity of CAT were measured by ELISA method. Results:TGF β1 enhanced the activity of pCOLH1.5 and pCOLH2.5(1.498 and 1.551 fold,respectively) significantly compared to the control; Verapamil inhibited the activity of the 2 recombinants. The activity of CAT stimulated by verapamil + TGF β1 was remarkably decreased compared with the activity of pCOLH2.5 promotor by TGF β1. Conclusion:Verapamil can not only inhibit the activity of gene promotor derectly, but also partly block the activity of α1(Ⅰ) collagen gene promotor induced by TGF β1.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2002年第8期868-870,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目 (39870 30 1)
