摘要
目的:研究枳实药材乙醇提取物的化学成分。方法:采用硅胶柱、开放ODS柱、制备液相色谱、HW-40F凝胶柱、大孔树脂柱对枳实药材乙醇提取物进行分离纯化,根据理化性质和波谱(质谱、氢谱、碳谱)数据分析鉴定化合物结构。结果:从枳实药材乙醇提取物中分离得到8个化合物,分别鉴定为Rimboxo(1)、胸苷(2)、尿嘧啶(3)、环-(亮氨酸1-异亮氨酸2-丙氨酸3-苏氨酸4-甘氨酸5-苏氨酸6-苯丙氨酸7)(4)、环-(亮氨酸1-亮氨酸2-脯氨酸3-酪氨酸4-甘氨酸5-丝氨酸6-脯氨酸7)(5)、水合橙皮内酯-β-D-葡萄糖苷(6)、伞形花内酯(7)、里那醇苷(8)。结论:化合物1、2、3、8为首次从柑橘属植物中分离得到,化合物4、5、6为首次从枳实药材中分离得到,该研究为枳实药材质量评价奠定了一定基础。
OBJECTIVE:To study the chemical constituents of ethanol extract from Cirtus aurantium. METHODS:The ethanol extract of C. aurantium was isolated and purified by silica gel column, open ODS column, preparation liquid chromatography,HW-40 F gel column and macroporous resin column. The structure of compounds was analyzed and identified according to physicochemical properties and spectral data(mass spectrum,hydrogen spectrum,carbon spectrum). RESULTS:Eight compounds were isolated from ethanol extract of C. aurantium,i.e. Rimboxo(1),Thymidine(2),Uracil(3),Cyclo-(Leu1-Ile2-Ala3-Thr4-Gly5-Thr6-Phe7)(4),Cyclo-(Leu1-Leu2-Pro3-Tyr4-Gly5-Ser6-Pro7)(5),Meranzin hydrate-β-D-glucoside(6),Umbelliferone(7),Linaloyl glucoside(8). CONCLUSIONS:Compound 1,2,3,8 are isolated from Citrus L. for the first time,compound 4,5,6 were isolated from C. aurantium for the first time. The study lays the foundation for quality evaluation of C.aurantium.
出处
《中国药房》
CAS
北大核心
2018年第4期458-460,共3页
China Pharmacy
基金
国家自然科学基金资助项目(No.81260621)
关键词
枳实
化学成分
结构鉴定
乙醇提取物
柑橘属
Cirtus aurantium
Chemical constituent
Structural identification
Ethanol extract
Citrus L
