摘要
以新分离的蝉花(Cordyceps cicadae)CC1504为试材,通过真菌核糖体DNA内转录间隔区(ITS)序列分析确定菌株的分类学地位;利用琼脂糖-纤维蛋白平板法测定蝉花产纤溶酶的能力,通过单因素试验确定其液体发酵产纤溶酶的最适碳源和最适氮源;以正交实验优化菌株产纤溶酶的培养基组成。结果表明:ITS序列进化树分析确定了该菌株的分类地位为蝉花;该蝉花产纤溶酶的最适碳源为乳糖,产酶水平为248.95U·mL^(-1),其次是甘油,为198.94U·mL^(-1);蝉花产纤溶酶的最适氮源为蛋白胨,产酶水平可达240.68U·mL^(-1),其次是酵母膏,为89.23U·mL^(-1);获得的最佳培养基碳氮源组成为:乳糖10g·L^(-1)、蛋白胨10g·L^(-1)、酵母膏3g·L^(-1),可发酵获得的纤溶酶水平达262.05U·mL^(-1)。
A newly isolated Cordyceps cicadae strain CC1504 was used as research material in this study.The taxonomy position of the fungus was confirmed by analysis of its internal transcribed spacer（ITS）sequences of ribosomal DNA.Agarose-fibrin plate method was employed to detect the fibrinolytic enzymes activity produced by the fungal strain.The carbon or nitrogen sources for the production of fibrinolytic enzymes were screened by single-factor experiments.Orthogonal experimental design was conducted to optimize the medium component for fibrinolytic enzymes production.The results indicated that the fungal strain CC1504 belonged to Cordyceps cicadae according to the phylogenetic tree analysis based on ITS sequences.The optimal carbon source was lactose for the production of fibrinolytic enzymes,and the highest production level up to 248.95 U·mL^（-1）,followed by glycerol,given the yield at 198.94 U·mL^（-1）.Peptone was the optimal nitrogen source for the production of enzymes,giving maximum level at 240.68 U·mL^（-1）,and followed by yeast extract with production level of 89.23 U · mL^（-1）.The optimized medium formula（carbon and nitrogen sources）was as：lactose 10 g·L^（-1）,peptone 10 g·L^（-1） and yeast extract 3 g·L^（-1）,giving the production of 262.05 U·mL^（-1）.
出处
《北方园艺》
CAS
北大核心
2018年第3期28-33,共6页
Northern Horticulture
基金
江苏省高校自然科学基金资助项目(15KJB220003)
南京林业大学大学生创新训练计划资助项目(2015sjcx033)
江苏省大学生科技创新计划资助项目(201610298104X)
关键词
蝉花
纤溶酶
营养条件
液体发酵
Cordyceps cicadae
fibrinolytic enzyme
nutritional sources
liquid culture
