摘要
目的探讨过表达miRNA-125b对肺癌细胞A549的增殖、侵袭能力的影响及其机制。方法将A549细胞分为3组:miRNA-125b组(miR-125b模拟物),NC组(NC模拟物)及空白组(同等体积的混有转染剂的胎牛血清),采用Q-PCR检测miRNA-125b的转染及表达效率。MTT法分析各组细胞的增殖能力,Transwell小室实验检测细胞的侵袭能力。采用Western blot法检测各组细胞Bcl-2调节因子(BMF)的表达水平。结果与空白组比较,miRNA-125b组细胞的miRNA-125b表达水平、增殖能力及侵袭能力上升(P<0.05);NC组的上述指标无明显变化(P>0.05)。与空白组比较,miRNA-125b组细胞的BMF表达水平下降(P<0.05);NC组的BMF表达水平无明显变化(P>0.05)。结论 miRNA-125b能通过抑制BMF的表达促进肺癌细胞A549的增殖及侵袭。
Objective To study the influence of miRNA-125b over-expression on proliferation and invasion ability of lung cancer A549 cells and its mechanism. Methods A549 cells were divided into 3 groups..miRNA-125b group（transfected with miR- NA-125b mimics）, NC group（transfected with NC mimics） and blank group（same volume of GIBCO serum mixed with transfection agent）. The transfection and expression efficiency of miRNA-125b was detected with Q-PCR,the proliferation ability was detected with MTT,and the invasion ability was detected with the transwell chamber test. The expression level of BMF in A549 cells was detected with Western blot. Results Compared with the blank group,the expression level of miRNA-125b,proliferation ability and invasion ability in the miRNA-125b group were increased（P〈0.05） while the above indexes in the NC group demonstrated no sig nificant change（P〈0.05）. Compared with the blank group, the expression level of BMF in the miRNA-125b group was decreased （P〈0.05） while which in the NC group had no significant ehange（P〈0.05）. Conclusion miRNA-125b can promote the prolifer ation and invasion ability of A549 cells via inhibiting the expression of BMF.
出处
《重庆医学》
CAS
2018年第5期604-606,共3页
Chongqing medicine
基金
珠海市科技计划项目(2015A1005)
